Prof. Dr. Nils Wiedemann

Biogenesis of mitochondrial membranes:

The mitochondrial membranes are crucial for oxidative phosphorylation and cell survival. Most of the mitochondrial membrane proteins and lipids are synthesized in the cytosol and the endoplasmic reticulum (ER) respectively. We analyze the ER-mitochondria encounter structure, which forms an inter-organellar connection, and the protein translocases in the inner and outer membrane required for the import of membrane proteins and lipids.

10 selected publications:

• Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
  Morgenstern M, Peikert CD, Lübbert P, Suppanz I, Klemm C, Alka O, Steiert C, Naumenko N, Schendzielorz A, Melchionda L, Mühlhäuser WWD, Knapp B, Busch JD, Stiller SB, Dannenmaier S, Lindau C, Licheva M, Eickhorst C, Galbusera R, Zerbes RM, Ryan MT, Kraft C, Kozjak-Pavlovic V, Drepper F, Sven Dennerlein S, Oeljeklaus S, Pfanner N, Wiedemann N* & Warscheid B* (2021).
  Cell Metabolism 33(12), 2464-2483.e18. (* corresponding)
• Mitochondrial sorting and assembly machinery operates by β-barrel switching.
  Takeda H, Tsutsumi A, Nishizawa T, Lindau C, Busto JV, Wenz L-S, Ellenrieder L, Imai K, Straub SP, Mossmann W, Qiu J, Yamamori Y, Tomii K, Suzuki J, Murata T, Ogasawara S, Nureki O, Becker T, Pfanner N, Wiedemann N, Kikkawa N, Endo, T. (2021).
  Nature 590(7844), 163–169.
• Structure of the mitochondrial import gate reveals distinct preprotein paths.
  Araiso Y, Tsutsumi A, Qiu J, Imai K, Shiota T, Song J, Lindau C, Wenz LS, Sakaue H, Yunoki K, Kawano S, Suzuki J, Wischnewski M, Schütze C, Ariyama H, Ando T, Becker T, Lithgow T, Wiedemann N, Pfanner N, Kikkawa M, Endo T (2019).
  Nature. 575(7782):395-401
• Structural basis of membrane protein chaperoning through the mitochondrial intermembrane space.
  Weinhäupl K, Lindau C, Hessel A, Wang Y, Schütze C, Jores T, Melchionda L, Schönfisch B, Kalbacher H, Bersch B, Rapaport D, Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P (2018).
  Cell. 175(5):1365-1379.e25
• Membrane protein insertion through a mitochondrial β-barrel gate.
  Höhr AIC, Lindau C, Wirth C, Qiu J, Stroud DA, Kutik S, Guiard B, Hunte C, Becker T, Pfanner N, Wiedemann N (2018).
  Science 359, eaah6834.
• Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale.
  Morgenstern M, Stiller SB, Lübbert P, Peikert CD, Dannenmeier S, Drepper F, Weill U, Höß P, Feuerstein R, Gebert M, Bohnert M, van der Laan M, Schuldiner M, Schütze C, Oeljeklaus S, Pfanner N, Wiedemann N*, Warscheid B* (2017).
  Cell Rep. 19, 2836–2852. (* corresponding)
• Mitochondrial OXA translocase plays a major role in biogenesis of inner-membrane proteins.
  Stiller SB, Höpker J, Oeljeklaus S, Schütze C, Schrempp SG, Vent-Schmidt J, Horvath SE, Frazier AE, Gebert N, van der Laan M, Bohnert M, Warscheid B, Pfanner N, Wiedemann N (2016).
  Cell Metabolism 23, 901–908.
• Coupling of Mitochondrial Import and Export Translocases by Receptor-Mediated Supercomplex Formation.
  Qiu J, Wenz L-S, Zerbes RM, Oeljeklaus S, Bohnert M, Stroud DA, Wirth C, Ellenrieder L, Thornton N, Kutik S, Wiese S, Schulze-Specking A, Zufall N, Chacinska A, Guiard B, Hunte C, Warscheid B, van der Laan M, Pfanner N*, Wiedemann N*, Becker T (2013).
  Cell 154, 596–608. (* corresponding)
• Dual Function of Sdh3 in the Respiratory Chain and TIM22 Protein Translocase of the Mitochondrial Inner Membrane.
  Gebert N, Gebert M, Oeljeklaus S, Malsburg von der K, Stroud DA, Kulawiak B, Wirth C, Zahedi RP, Dolezal P, Wiese S, Simon O, Schulze-Specking A, Truscott KN, Sickmann A, Rehling P, Guiard B, Hunte C, Warscheid B, van der Laan M, Pfanner N, Wiedemann N (2011).
  Mol Cell 44, 811–818.
• Dissecting membrane insertion of mitochondrial β-barrel proteins.
  Kutik S, Stojanovski D, Becker L, Becker T, Meinecke M, Krüger V, Prinz C, Meisinger C, Guiard B, Wagner R, Pfanner N and Wiedemann N (2008).
  Cell 132, 1011-1024.