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Prof. Pascal Tomakidi
Prof. Pascal Tomakidi
Oral Biotechnology
University Medical Center Freiburg
The work of our group aims at understanding how biomechanical cues of the extracellular environment, e.g. micropatterning of cell adhesion points and substrate/matrix stiffness/elasticity govern cell behaviour, in order to maintain or modulate cell and tissue homeostasis.
To this end, identification and characterization of mechano-sensitive molecules is indispensible. In this context, molecules of particular importance address cell functions including (i) mechanopercetion, (ii) signal-/mechanotransduction, and (iii) execution of mechano-response. To realize this aim, we employ simple monolayer cultures and/or interactive co-cultures of tissue-relevant cells, considering physiological, i.e. soluble growth factor-orchestrated in vivo-relevant cell-to-cell-interactions. Tissues addressed include epithelia in conjunction with connective tissue counterparts of oral cavity and skin, the periodontal ligament as well as the alveolar bone, but also cornea. As a prospective goal, we focus on translation of the experimental progressive elucidation of biomechanics in mechano-signalling terms into material science, i.e. the creation of new tissue-supporting biomaterials.
10 seleceted publications
- Molecular Research on Oral Diseases and Related Biomaterials: A Journey from Oral Cell Models to Advanced Regenerative Perspectives.
Steinberg T, Dieterle MP, Tomakidi P (2022).
Int J Mol Sci. 23(9):5288. - FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration.
Wang X, Steinberg T, Dieterle MP, Ramminger I, Husari A, Tomakidi P (2021).
Int J Mol Sci. 22(18):9774. - From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues.
Dieterle MP, Husari A, Steinberg T, Wang X, Ramminger I, Tomakidi P (2021).
Biomolecules. 11(6):824. - Role of Mechanotransduction in Periodontal Homeostasis and Disease.
Dieterle MP, Husari A, Steinberg T, Wang X, Ramminger I, Tomakidi P (2021).
J Dent Res. 100(11):1210-1219. - Force-responsive Zyxin modulation in periodontal ligament cells is regulated by YAP rather than TAZ.
Belgardt E, Steinberg T, Husari A, Dieterle MP, Hülter-Hassler D, Jung B, Tomakidi P (2020).
Cell Signal. 72:109662. - On the relationship of YAP and FAK in hMSCs and osteosarcoma cells: Discrimination of FAK modulation by nuclear YAP depletion or YAP silencing.
Husari A, Steinberg T, Dieterle MP, Prucker O, Rühe J, Jung B, Tomakidi P (2029).
Cell Signal. 63:109382. - Gelatin nonwovens-based epithelial morphogenesis involves a signaling axis comprising EGF-receptor, MAP kinases ERK 1/2, and β1 integrin.
Jedrusik N, Steinberg T, Husari A, Volk L, Wang X, Finkenzeller G, Strassburg S, Tomakidi P (2019).
J Biomed Mater Res A. 107(3):663-677. - Nanofibered Gelatin-Based Nonwoven Elasticity Promotes Epithelial Histogenesis.
Jedrusik N, Meyen C, Finkenzeller G, Stark GB, Meskath S, Schulz SD, Steinberg T, Eberwein P, Strassburg S, Tomakidi P (2018).
Adv Healthc Mater. 7(10):e1700895. - Disruption of adherens junction and alterations in YAP-related proliferation behavior as part of the underlying cell transformation process of alcohol-induced oral carcinogenesis.
Husari A, Hülter-Hassler D, Steinberg T, Schulz SD, Tomakidi P (2018).
Biochim Biophys Acta Mol Cell Res. 1865(1):209-219. - Biomechanical strain-induced modulation of proliferation coincides with an ERK1/2-independent nuclear YAP localization.
Hülter-Hassler D, Wein M, Schulz SD, Proksch S, Steinberg T, Jung BA, Tomakidi P(2017).
Exp Cell Res. 361(1):93-100.