Centrifugal microfluidic system for primary amplification and secondary real-time PCR
11.10.2010
Focke M, Stumpf F, Roth G, Zengerle R, von Stetten F.
Lab Chip 10(23):3210-2
Pre-amplification is a basis for numerous polymerase chain reaction (PCR) protocols but bears severe contamination risks due to handling of high-copy DNA samples. Therefore we developed a selfcontained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification of specific DNA sequences prior to automated aliquoting and real-time PCR in a modified commercial thermocycler.
