Aliquoting on the centrifugal microfluidic platform based on centrifugo-pneumatic valves

08.01.2011

Mark D, Weber P, Lutz S, Focke M, Zengerle R, von Stetten F

Microfluid Nanofluid (2011) 10:1279–1288

Microfluid Nanofluid        online article

Aliquoting is an essential liquid handling step to perform multiple parallel assays (“geometric multiplexing”) from one sample, such as genotyping by real-time PCR. Our microfluidic solution for this task includes precise metering and the possibility to pre-store reagents without influencing the metering precision. It excludes any cross-contamination between the receiving cavities. We characterized the performance for micro-milling, thermoforming of foils, and injection molding.