Conditional DNA-protein interactions confer stimulus-sensing properties to biohybrid materials
25.05.2011
Christen E, Karlsson M, Kämpf MM, Schoenmakers R, Gübeli RJ, Wischhusen HM, Friedrich C, Fussenegger M, Weber W
Adv Func Mat 2011, 21, 2861–2867
In this study we synthesize a biohybrid hydrogel for the drug-inducible release of biopharmaceuticals. The hydrogel consists of linear polyacrylamide crosslinked by the interaction of the tetracycline repressor TetR with its target DNA sequence tetO. Addition of tetracycline dissociates the protein-DNA interaction and triggers the release of a previously embedded payload protein.
