Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases.

28.05.2011

Csorba K, Schmidt S, Florea F, Ishii N, Hashimoto T, Hertl M, Karpati S, Bruckner-Tuderman L, Nishie W, Sitaru C

Orphanet J Rare Dis. 2011;6(1):31

Orphanet J Rare Dis     online article

In pemphigoids, a group of autoimmune skin blistering diseases collagen XVII/BP180 is a major target of autoantibodies (aAbs) belonging to the IgG, IgE and IgA isotypes. For the detectionn of BP 180-specific circulating IgG and IgE aAbs several ELISAs were developed. In the present study we used a recombinant form of BP 180 ectodomain to establish the first sensitive and specific ELISA for the detection of BP 180-specific IgA aAbs from sera of patients with the lamina lucida-type of linear IgA disease (n= 30) and healthy donors (n=105). The test´s specificity was 83.3%. This new ELISA should prove a useful tool for clinical and translational research as well as for the diagnosis and disease monitoring of patients with IfA pemphigiod.