Pou5f1 contributes to dorsoventral patterning by positive regulation of vox and modulation of fgf8a expression
20.05.2011
Belting HG, Wendik B, Lunde K, Leichsenring M, Mössner M, Driever W, Onichtchouk D
Dev Biol 2011; 356; 323–336
A contribution of Pou5f1 to the control of bmp2b, bmp4 and vox expression has been suggested, however the mechanisms remained unclear and are investigated in detail here. We show that Pou5f1 binds to phylogenetically conserved Oct/Pou5f1 sites in the vox promoter, both in vivo (ChIP) and in vitro. Our data reveals a set of direct and indirect interactions of Pou5f1 with the BMP dorsoventral patterning network that serve to fine-tune dorsoventral patterning mechanisms and coordinate patterning with developmental timing.
