Evaluation of Bicinchoninic Acid as Ligand for Copper(I)-catalyzed Azide-Alkyne Bioconjugations

05.07.2012

Christen EH, Gübeli RJ, Kaufmann B, Merkel L, Schoenemakers R, Budisa N, Fussenegger M, Weber W, Wiltschi B

Org Biomol Chem. 2012;10(33):6629-32

Org Biomol Chemistry            online article

The Cu(I)-catalyzed cycloaddition of terminal azides and alkynes (click chemistry) represents a highly specific reaction for the functionalization of biomolecules with chemical moieties such as dyes or polymer matrices. We demonstrate that the BCA-Cu(I)-complex represents an efficient catalyst for the conjugation of fluorophores or biotin to alkyne- or azide-functionalized proteins resulting in increased or at least equal reaction yields compared to commonly used catalysts.