Release probability of hippocampal glutamatergic terminals scales with the size of the active zone.
10.06.2012
Holderith N, Lorincz A, Katona G, Rózsa B, Kulik A, Watanabe M, Nusser Z.
Nat Neurosci. 2012 Jun 10;15(7):988-97
Two-photon Ca2+ imaging-derived optical quantal analysis and correlated electron microscopic reconstruction revealed a tight correlation between the release probability and the active-zone area. Freeze-fracture immunogold labeling
revealed that the voltage-gated calcium channel subunit Cav2.1 and the presynaptic protein Rim1/2 are confined to the active zone and their numbers scale linearly with the active zone area. Gold particles labeling Cav2.1 were nonrandomly distributed in the active zones. Our results demonstrate that the number of presynaptic calcium channels as well as the number of docked vesicles and the release probability scale linearly with the active-zone area.
