Universal protocol for grafting PCR primers onto various Lab-ona-Chip substrates for solid-phase PCR
13.03.2012
Hoffmann J, Hin S, von Stetten F, Zengerle R, Roth G
RSC Advances 2012, DOI: 10.1039/C2RA01250B
A universal protocol for grafting PCR primers onto glass, PDMS, COP, COC, and PP is developed and evaluated by solid-phase PCR (SP-PCR). Primers are immobilized featuring spots with high homogeneity. Furthermore, we show a protocol for binding a PCR product to immobilized PCR primers via SP-PCR. The presented immobilization- and SP-PCR protocols may enable to integrate DNA microarrays directly into microfluidic Lab-on-a-Chip cartridges of various materials for analysis via SP-PCR.
