Centrifugal gas-phase transition magnetophoresis (GTM) - a generic method for automation of magnetic bead based assays on the centrifugal microfluidic platform and application to DNA purification.
12.11.2012
Strohmeier O, Emperle A, Roth G, Mark D, Zengerle R, von Stetten F.
Lab Chip. 2013; 13(1):146-55
A facile and automated method for the transportation of magnetic beads between multiple microfluidic chambers on a centrifugal microfluidic cartridge “LabDisk” is presented. The method requires only one stack of stationary permanent magnets, a specific microfluidic layout without actively controlled valves and a predefined frequency protocol for 15 rotation of the LabDisk. Magnetic beads were transported through three fluidically separated chambers with a yield of 82.6% ± 3.6%. Bead based DNA purification was demonstrated where the three chambers were used for binding, washing and elution of DNA.
