Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks
30.01.2013
Boerries M, Grahammer F, Eiselein S, Buck M, Meyer C, Goedel M, Bechtel W, Zschiedrich S, Pfeifer D, Laloë D, Arrondel C, Gonçalves S, Krüger M, Harvey SJ, Busch H, Dengjel J, Huber TB.
Kidney Int. 2013;83(6):1052-64
A thorough characterization of the transcriptome and proteome of endogenous podocytes has been hampered by low cell yields. Here we describe an optimized podocyte isolation procedure allowing for global, unbiased downstream
applications. Combining mRNA and miRNA transcriptional profiling with quantitative proteomic analyses revealed programs of highly specific gene regulation tightly controlling cytoskeleton, cell differentiation, endosomal transport, and peroxisome function in podocytes.
