Rapid combinatorial ERLIC-SCX solid-phase extraction for in-depth phosphoproteome analysis

04.11.2013

Zarei M, Sprenger A, Gretzmeier C, Dengjel J

J Proteome Res. 2013;12(12):5989-95

J Proteome Res.          online article

Here we present a simple and rapid strategy implemented as solid-phase extraction not requiring specific instrumentation, such as off-line HPLC systems, for global phosphoproteomics approaches. It is inexpensive, adaptable for high and low amounts of starting material, and saves time by allowing multiplexed sample preparation without any carry-over problem.