SMC condensation centers in Bacillus subtilis are dynamic structures
08.03.2013
Kleine Borgmann LA, Hummel H, Ulbrich MH, Graumann PL
J Bacteriol. 2013;195(10):2136-45
SMC and MukB complexes consist of a central dimer and two essential binding partners, ScpA and ScpB, and are crucial for chromosome compaction and segregation. The complexes form bipolar assemblies on the chromosome. Using fluorescence recovery after photobleaching, we provide evidence that SMC has high exchange rates. Our experiments reveal that SMChas a high turnover rate and that an alteration of the ATPase cycle affects SMC function in vivo, while nucleoid-associated proteins feature slow exchange between different sites on the nucleoid, which may be the basis for epigenetic-like phenomena observed in bacteria.
