A Pou5f1/Oct4 dependent Klf2a, Klf2b, and Klf17 regulatory sub-network contributes to EVL and ectoderm development during zebrafish embryogenesis
05.11.2013
Kotkamp K, Mössner R, Allen A, Onichtchouk D, Driever W.
Dev Biol. 2014;385(2):433-47
In mammalian ES cells, the Oct4-dependent Klf transcription factors contribute to maintenance of pluripotency. In the early zebrafish embryo, Pou5f1/Oct4 acts in combination with region-specific factors to activate klf2a, klf2b, and klf17 in the superficial cell layer of the embryo. In addition, Pou5f1 acts together with the BMP signaling pathway to activate and maintain expression of klf2a and klf2b in a ventral ectodermal domain. Microarray expression profiles of klf2a, klf2b and klf17 knockdown and overexpression embryos identify Klf target genes, which reveals that Klfs participate in specification of the extraembryonic enveloping layer.
