Pou5f1 protein expression and posttranslational modification during early zebrafish development
21.11.2013
Lippok B, Song S, Driever W.
Dev Dyn. 2014;243(3):468-77
Pou5f1/Oct4 is a transcription factor essential for maintenance of pluripotency in mammals and control of blastula and gastrula stage gene regulatory networks in zebrafish. We show that Pou5f1 protein is present in unfertilized oocytes, and persists in all embryonic and enveloping layer cell nuclei until the end of gastrulation. The developmental protein profile correlates with the stages during which deep cells are not committed to a specific germ layer. Pou5f1 is subject to multiple developmentally regulated phosphorylation, suggesting temporal or spatial modulation of its activity by embryonic signaling mechanisms.
