Real-time PCR based detection of a panel of foodborne pathogens on a centrifugal microfluidic “LabDisk” with on-disk quality controls and standards for quantification
06.01.2014
Strohmeier O, Marquart N, Mark D, Roth G, Zengerle R, von Stetten F
Anal. Methods 2014,6, 2038-2046
We present an implementation of parallel, real-time PCR based detection of up to 6 different food-borne pathogens on our centrifugal microfluidic “LabDisk” platform. It has the following two novelties: (1) a microfluidic network for integration of positive controls (PCs), no-template controls (NTCs), and standards (STDs) into a centrifugal microfluidic PCR cartridge; (2) a microfluidic unit operation for sequential aliquoting of two liquids of highly different wetting characteristics into fourteen aliquots with 5.8 µL +/- 0.3 µL (PCR mastermix) and 6.1+/- 0.8 µL (elution buffer), respectively. The presented “LabDisk” implementation can be used either in a qualitative or quantitative operation mode depending on the prestorage scheme of reagents.
