Targeted purification of SnAvi-tagged proteins.

29.05.2014

Schäffer U, Baumeister R, Schulze E.

Methods Mol Biol. 2014;1177:163-74.

Methods Mol Biol        online article

Tandem affinity purifi cation (TAP) is a powerful technique to identify protein complex members. The modular composition of TAP-tags allows two sequential protein enrichment steps and thereby drastically reduces the amount of contaminants. Here, we describe the application of the SnAvi-tag—a TAP-tag useful in different expression systems. Due to its modular composition, this tag is multifunctional and facilitates among others the in vivo visualization of tagged proteins and their cell type specific activation.