A peptide-functionalized polymer as a minimal scaffold protein to enhance cluster formation in early T cell signal transduction.
06.02.2015
Witsenburg JJ, Sinzinger MD, Stoevesandt O, Ruttekolk IR, Roth G, Adjobo-Hermans MJ, Brock R.
Chembiochem. 2015;16(4):602-10.
We applied microcontact printing to enable cell signalling of T-cells on a sub-cellular level (as the activating structures have been smaller als the cells). Here, we explored the minimal structural requirement for a scaffold protein by coupling multiple copies of a proline-rich peptide corresponding to an interaction motif for the SH3 domain of the adaptor protein GADS to a N-(2-hydroxypropyl)- methacrylamide polymer backbone.
