Analysis of fast protein phosphorylation kinetics in single cells on a microfluidic chip
27.11.2014
Blazek M, Santisteban TS, Zengerle R, Meier M.
Lab Chip. 2015;15(3):726-34
We developed a microfluidic large-scale integration (mLSI) platform for the temporal and chemical control of cell cultures to study fast kinetics of protein phosphorylation. For in situ protein analysis the mLSI chip integrates the Proximity Ligation Assay (PLA). To investigate cell-signaling events with a time resolution of a few seconds we first engineered and optimized the fluidic layout of the chip with 128 individual addressable cell culture chambers. Functionality is demonstrated by the determination of the minimum cell sample size for obtaining robust quantitative PLA signals at the single-cell level.
