Automated nucleic acid extraction from whole blood, B . subtilis, E. coli, and Rift Valley fever virus on a centrifugal microfluidic LabDisk
27.03.2015
Strohmeier O, Keil S, Kanat B, Patel P, Niedrig M, Weidmann M, Hufert F, Drexler J, Zengerle R, von Stetten F
RSC Adv., 2015,5, 32144-32150
We present total nucleic acid extraction from whole blood, Gram?positive Bacillus subtilis, Gram?negative Escherichia coli, and Rift Valley fever RNA virus on a low?cost, centrifugal microfluidic LabDisk cartridge processed in a light?weight and portable processing device. Compared to earlier work in disk based centrifugal microfluidics, this includes the following advances: combined lysis and nucleic acid purification on one cartridge and handling of sample volumes as large as 200 ?L.
