Digital droplet PCR on disk
09.11.2015
Schuler F, Trotter M, Geltman M, Schwemmer F, Wadle S, Dominguez-Garridod E, Lopezd M, Cervera-Acedod C, Santibanezd P, von Stetten F, Zengerle R, Paust N
Labchip 2015; DOI: 10.1039/C5LC01068C
Existing systems for digital droplet PCR (ddPCR) either suffer from low integration or are difficult to introduce to mass fabrication. Here we present an integrated system that is compatible to mass fabrication and combines emulsification, PCR, and fluorescence readout in a single chamber within a disposable cartridge (disk). We envision that this work will lay the base for the development of highly integrated sample-to-digital-answer PCR systems that can be employed in routine clinical diagnosis.
