Enhanced single-cell printing by acoustophoretic cell focusing

31.03.2015

Leibacher I, Schoendube J, Dual J, Zengerle R, Koltay P

Biomicrofluidics. 2015;9(2):024109.

Biomicrofluidics             online article

To access and handle single-cells, many new tools are needed and have partly been developed. Here, we present an improved version of a single-cell printer which is able to deliver individual single cells and beads encapsulated in free-flying picoliter droplets at a single-bead efficiency of 96% and with a throughput of more than 10 beads per minute. By integration of acoustophoretic focusing, the cells could be focused in x and y direction.