Microfluidic proximity ligation assay for profiling signaling networks with single-cell resolution

Blazek M, Roth G, Zengerle R, Meier M.

Methods Mol Biol. 2015;1346:169-84.

Methods Mol Biol.            online article

The proximity ligation assay (PLA) is a technique that can be used to characterize proteins, protein -protein interactions, and protein modifications at the single-cell level. Image-based in situ detection of proteins using PLA is a quantitative method with a high degree of sensitivity and specificity. The miniaturization and parallelization of the PLA onto a microfluidic chip and concurrent use of an automated cell-culture system increase the throughput of this technology. Here, we describe the performance of PLA on a microfluidic chip.