Single-cell printing based on impedance detection

01.01.2015

Schoendube J, Wright D, Zengerle R, Koltay P

Biomicrofluidics. 2015 Feb 11;9(1):014117

Biomicrofluidics           online article

Label-free isolation of single cells is essential for the growing field of single-cell analysis. Here, we resent a device which prints single living cells encapsulated in free-flying picoliter droplets. It combines inkjet printing and impedance flow cytometry. Droplet volume can be controlled in the range of 500 pl -800 pl by piezo actuator displacement. Viability of printed HeLa cells and human primary fibroblasts was demonstrated by culturing cells for at least eight days.