One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy
Yofe I, Weill U, Meurer M, Chuartzman S, Zalckvar E, Goldman O, Ben-Dor S, Schütze C, Wiedemann N, Knop M, Khmelinskii A, Schuldiner M.
Nat Methods. 2016;13(4):371-8.
The yeast Saccharomyces cerevisiae is ideal for systematic studies relying on collections of modified strains. Despite the significance only a few such libraries exist, as their construction is extremely expensive and laborious. To overcome these limitations, we developed a SWAp-Tag (SWAT) method that enables one parental library to be modified easily and efficiently to give rise to an endless variety of libraries of choice.
