LabDisk with complete reagent prestorage for sample-to-answer nucleic acid based detection of respiratory pathogens verified with influenza A H3N2 virus.
25.11.2015 Stumpf F, Schwemmer F, Hutzenlaub T, Baumann D, Strohmeier O, Dingemanns G, Simons G, Sager C, Plobner L, von Stetten F, Zengerle R, Mark D. Lab Chip. 2016;16(1):199-207. In this work we present a fully automated sample-to-answer detection of influenza A H3N2 virus in a centrifugal LabDisk with complete prestorage of reagents. Thus, the initial supply of the sample remains the only manual handling step. The self-contained LabDisk automates by centrifugal microfluidics all necessary process chains for PCR-based pathogen detection. All experiments were applied in a 2 kg portable, laptop controlled point-of-care device. The turnaround time of the complete analysis from sample-to-answer was less than 3.5 hours. 