The single berberine bridge enzyme homolog of Physcomitrella patens is a cellobiose oxidase
Toplak M, Wiedemann G, Ulićević J, Daniel B, Hoernstein SNW, Kothe J, Niederhauser J, Reski R, Winkler A, Macheroux P.
FEBS J. 2018;285(10):1923-1943.
Berberine bridge enzymes (BBE) are involved in the biosynthesis of benzylisoquinoline alkaloids. Here we cloned and expressed PpBBE1 of Physcomitrella patens, the most basal land plant harboring a BBE enzyme. In this study we performed biochemical, structural, and in vivo characterization of PpBBE1. Catalytic residues in the active site were identified using a structure-guided mutagenesis approach. Our results suggest a role of BBE-like enzymes in primary metabolic reactions and signaling of carbohydrate utilization.
