Physcomitrella patens Reute mCherry as a tool for efficient crossing within and between ecotypes

Plant Biol.                   online article

P. patens is a monoecious moss that is predominantly selfing in the wild. Using newly established Reute fluorescent strains, we show that they can efficiently fertilise different ecotypes. The resulting progeny display Mendelian 1:1 segregation of the fluorescent marker(s), demonstrating the suitability of such strains for genetic crossing. Overall our results demonstrate that ecotype Reute is highly suitable for genetic crossing. The Reute mCherry strain can be used as a suitable background for offspring selection after crossing.