Actin polymerization regulates recruitment of Nck to CD3ε upon T-cell receptor triggering
Wipa P, Paensuwan P, Ngoenkam J, Woessner NM, Minguet S, Schamel WW, Pongcharoen S
In our Excellence Cluster project we study signaling by the T cell receptor (TCR). Following TCR stimulation, rearrangement of the actin cytoskeleton supports intracellular signal transduction and T-cell activation. Implicatedin this ist he adaptor protein Nck. Previously we have shown that upon TCR triggering Nck is recruited to the CD3e subunit of the TCR. In a collaboration with our friends in Thailand we examined the role of actin polymerization in the recruitment of Nck to the TCR. Using the proximity ligation assay, we show that inhibition of actin polymerization delayed the recruitment of Nck to the TCR upon TCR triggering. Interestingly, CD3e phosphorylation was also delayed. These findings suggest that actin polymerization and phosphorylation of the TCR are more interwoven than previously thought.
