2024
- From TCR fundamental research to innovative chimeric antigen receptor design.
Minguet S, Maus MV, Schamel WW.
Nat Rev Immunol. 2024 Oct 21. doi: 10.1038/s41577-024-01093-7. Online ahead of print. - PD-1 and LAG-3: synergistic fostering of T cell exhaustion.
Hofmann M, Thimme R, Schamel WW.
Signal Transduct Target Ther. 2024 Oct 18;9(1):291. - TCR/CD3-based synthetic antigen receptors (TCC) convey superior antigen sensitivity combined with high fidelity of activation.
Mühlgrabner V, Peters T, Velasco Cárdenas RM, Salzer B, Göhring J, Plach A, Höhrhan M, Perez ID, Goncalves VDR, Farfán JS, Lehner M, Stockinger H, Schamel WW, Schober K, Busch DH, Hudecek M, Dushek O, Minguet S, Platzer R, Huppa JB.
Sci Adv. 2024 Sep 6;10(36):eadj4632. - Sodium chloride in the tumor microenvironment enhances T cell metabolic fitness and cytotoxicity.
Soll D, Chu CF, Sun S, Lutz V, Arunkumar M, Gachechiladze M, Schäuble S, Alissa-Alkhalaf M, Nguyen T, Khalil MA, Garcia-Ribelles I, Mueller M, Buder K, Michalke B, Panagiotou G, Ziegler-Martin K, Benz P, Schatzlmaier P, Hiller K, Stockinger H, Luu M, Schober K, Moosmann C, Schamel WW, Huber M, Zielinski CE.
Nat Immunol. 2024 Oct;25(10):1830-1844. - The potential of γδ CAR and TRuC T cells: An unearthed treasure.
Schamel WW, Zintchenko M, Nguyen T, Fehse B, Briquez PS, Minguet S.
Eur J Immunol. 2024 Nov;54(11):e2451074. - Perinatal thymic-derived CD8αβ-expressing γδ T cells are innate IFN-γ producers that expand in IL-7R–STAT5B-driven neoplasms
Sumaria N*, Fiala GJ*, Inácio D, Curado-Avelar M, Cachucho A, Pinheiro R, Wiesheu R, Kimura S, Courtois L, Blankenhaus B, Darrigues J, Suske T, Almeida ARM, Minguet S, Asnafi V, Lhermitte L, Mullighan CG, Coffelt SB, Moriggl R, Barata JT*, Pennington DJ* & Silva-Santos B*
Nat Immunol. 2024 Jul;25(7):1207-1217. - Pro- and antitumorigenic functions of γδ T cells
Fiala GJ*, Lücke J*, Huber S.
Eur J Immunol. 2024 May 27;0:2451070 - Phospho-mimetic CD3ε variants prevent TCR and CAR signaling
Woessner NM, Brandl SM, Hartmann S, Schamel WW, Hartl FA*, Minguet S*.
Front Immunol 2024 May 08; 15:1392933. - Empowering γδ T-cell functionality with vitamin C.
Kabelitz D, Cierna L, Juraske C, Zarobkiewicz M, Schamel WW, Peters C.
Eur J Immunol. 2024 Apr 15;e2451028. - Type I Interferon, Induced by Adenovirus or Adenoviral Vector Infection, Regulates the Cytokine Response to Lipopolysaccharide in a Macrophage Type-Specific Manner.
Maler MD, Zwick S, Kallfass C, Engelhard P, Shi H, Hellig L, Zhengyang P, Hardt A, Zissel G, Ruzsics Z, Jahnen-Dechent W, Martin SF, Nielsen PJ, Stolz D, Lopatecka J, Bastyans S, Beutler B, Schamel WW, Fejer G, Freudenberg MA.
J Innate Immun. 2024;16(1):226-247. - Kidins220 and Aiolos promote thymic iNKT cell development by reducing TCR signals.
Herr LA, Fiala GJ, Schaffer AM, Hummel JF, Zintchenko M, Raute K, Velasco Cárdenas RMH, Heizmann B, Ebert K, Fehrenbach K, Janowska I, Chan S, Tanriver Y, Minguet S, Schamel WW.
Sci Adv 2024 Mar 15;10(11):eadj2802. - Mutation-specific CAR T cells as precision therapy for IGLV3-21R110 expressing high-risk chronic lymphocytic leukemia.
Märkl F, Schultheiß C, Ali M, Chen SS, Zintchenko M, Egli L, Mietz J, Chijioke O, Paschold L, Spajic S, Holtermann A, Dörr J, Stock S, Zingg A, Läubli H, Piseddu I, Anz D, Minden MD, Zhang T, Nerreter T, Hudecek M, Minguet S, Chiorazzi N, Kobold S, Binder M.
Nat Commun. 2024 Feb 2;15(1):993. - Dual targeting of cancer metabolome and stress antigens affects transcriptomic heterogeneity and efficacy of engineered T cells.
Hernández-López P, van Diest E, Brazda P, Heijhuurs S, Meringa A, Hoorens van Heyningen L, Riillo C, Schwenzel C, Zintchenko M, Johanna I, Nicolasen MJT, Cleven A, Kluiver TA, Millen R, Zheng J, Karaiskaki F, Straetemans T, Clevers H, de Bree R, Stunnenberg HG, Peng WC, Roodhart J, Minguet S, Sebestyén Z, Beringer DX, Kuball J.
Nat Immunol. 2024 Jan;25(1):88-101 - OptoREACT: Optogenetic Receptor Activation on Nonengineered Human T Cells.
Armbruster A, Ehret AK, Russ M, Idstein V, Klenzendorf M, Gaspar D, Juraske C, Yousefi OS, Schamel WW, Weber W, Hörner M.
ACS Synth Biol. 2024 Feb 9. doi: 10.1021/acssynbio.3c00518. - Kidins220 regulates the development of B cells bearing the λ light chain.
Schaffer AM, Fiala GJ, Hils M, Natali E, Babrak L, Herr LA, Romero-Mulero MC, Cabezas-Wallscheid N, Rizzi M, Miho E, Schamel WWA, Minguet S.
Elife. 2024 Jan 25;13:e83943. - Reprogramming of human γδ T cells by expression of an anti-CD19 TCR fusion construct (εTRuC) to enhance tumor killing
Juraske C, Krissmer SM, Teuber ES, Parigiani MA, Strietz J, Wesch D, Kabelitz D, Minguet S, Schamel WW
J Leukoc Biol. 2024 Jan 19;115(2):293-305 - Vdelta1 T cells are more resistant than Vdelta2 T cells to the immunosuppressive properties of galectin-3.
Schadeck J, Oberg HH, Peipp M, Hedemann N, Schamel WW, Bauerschlag D, Wesch D. Front Immunol. 2024 Jan 8;14:1286097. - A partial human LCK defect causes a T cell immunodeficiency with intestinal inflammation.
Lui VG, Hoenig M, Cabrera-Martinez B, Baxter RM, Garcia-Perez JE, Bailey O, Acharya A, Lundquist K, Capera J, Matusewicz P, Hartl FA, D’Abramo M, Alba J, Jacobsen EM, Niewolik D, Lorenz M, Pannicke U, Schulz AS, Debatin KM, Schamel WW, Minguet S, Gumbart JC, Dustin ML, Cambier JC, Schwarz K, Hsieh EWY.
J Exp Med. 2024 Jan 1;221(1):e20230927.
2023
- Combined Immunodeficiency Caused by a Novel Nonsense Mutation in LCK.
Keller B, Kfir-Erenfeld S, Matusewicz P, Hartl F, Lev A, Lee YN, Simon AJ, Stauber T, Elpeleg O, Somech R, Stepensky P, Minguet S, Schraven B, Warnatz K.
J Clin Immunol. 2023 Dec 19;44(1):4. - Harnessing CD3 diversity to optimize CAR T cells
Velasco Cárdenas RMH, Brandl SM, Meléndez AV*, Schlaak AE*, Buschky A, Peters T, Beier F, Serrels B, Taromi S, Raute K, Hauri S, Gstaiger M, Lassmann S, Huppa JB, Boerries M, Andrieux G, Bengsch B, Schamel WW, Minguet S
Nat Immunol. 2023 Dec;24(12):2135-2149 - A distinct topology of BTN3A IgV and B30.2 domains controlled by juxtamembrane regions favors optimal human γδ T cell phosphoantigen sensing
Karunakaran KM, Subramanian H, Jin Y, Mohammed F, Kimmel B, Juraske C, Starick L, Nöhren A, Länder N, Willcox CR, Singh R, Schamel WW, Nikolaev VO, Kunzmann V, Andrew J Wiemer AJ, Willcox BE, Herrmann T
Nat Commun. 2023 Nov 22;14(1):7617. - Engineering of an Optogenetic T Cell Receptor Compatible with Fluorescence-Based Readouts.
Idstein V, Ehret AK, Yousefi OS, Schamel WW.
ACS Synth Biol. 2023 Oct 20;12(10):2857-2864 - Exhaustion-associated cholesterol deficiency dampens the cytotoxic arm of antitumor immunity.
Yan C, Zheng L, Jiang S, Yang H, Guo J, Jiang LY, Li T, Zhang H, Bai Y, Lou Y, Zhang Q, Liang T, Schamel W, Wang H, Yang W, Wang G, Zhu ZJ, Song BL, Xu C.
Cancer Cell. 2023 Jul 10;41(7):1276-1293.e11 - Breast Cancer Stem Cell-Derived Tumors Escape from γδ T-cell Immunosurveillance In Vivo by Modulating γδ T-cell Ligands.
Raute K, Strietz J*, Parigiani MA*, Andrieux G, Thomas OS, Kistner KM, Zintchenko M, Aichele P, Hofmann M, Zhou H, Weber W, Boerries M, Swamy M, Maurer J, Minguet S.
Cancer Immunol Res. 2023 Jun 2;11(6):810-829. - MDM2 inhibition enhances immune checkpoint inhibitor efficacy by increasing IL-15 and MHC class II production.
Langenbach M, Giesler S, Richtsfeld S, da Costa-Pereira S, Rindlisbacher L, Wertheimer T, Braun LM, Andrieux G, Duquesne S, Pfeifer D, Woessner NM, Menssen HD, Taromi S, Duyster J, Boerries M, Brummer T, Blazar BR, Minguet S, Turko P, Levesque MP, Becher B, Zeiser R.
Mol Cancer Res. 2023 Aug 1;21(8):849-864. - Opto-APC: Engineering of cells that display phytochrome B on their surface for optogenetic studies of cell-cell interactions.
Russ M*, Ehret AK*, Hörner M, Peschkov D, Bohnert R, Idstein V, Minguet S, Weber W, Lillemeier BF, Yousefi OS, Schamel WW.
Front Mol Biosci. 2023 Feb 20;10:1143274.
2022
- Human β-defensin 2 ameliorates acute GVHD by limiting ileal neutrophil infiltration and restraining T cell receptor signaling.
Rückert T, Andrieux G, Boerries M, Hanke-Müller K, Woessner NM, Doetsch S, Schell C, Aumann K, Kolter J, Schmitt-Graeff A, Schiff M, Braun LM, Haring E, Kissel S, Siranosian BA, Bhatt AS, Nordkild P, Wehkamp J, Jensen BAH, Minguet S, Duyster J, Zeiser R, Köhler N.
Sci Transl Med. 2022 Dec 21;14(676):eabp9675. - Novel lectin-based chimeric antigen receptors target Gb3-positive tumour cells.
Meléndez AV, Velasco Cárdenas RM, Lagies S, Strietz J, Siukstaite L, Thomas OS, Tomisch J, Weber W, Kammerer B, Römer W, Minguet S.
Cell Mol Life Sci. 2022 Sep 12;79(10):513 - A Cysteine Residue within the Kinase Domain of Zap70 Regulates Lck Activity and Proximal TCR Signaling.
Schultz A, Schnurra M, El-Bizri A, Woessner NM, Hartmann S, Hartig R, Minguet S, Schraven B, Simeoni L.
Cells. 2022 Sep 1;11(17):2723. - The role of the different CD3γ domains in TCR expression and signaling.
Garcillán B, Megino RF, Herrero-Alonso M, Guardo AC, Perez-Flores V, Juraske C, Idstein V, Martin-Fernandez JM, Geisler C, Schamel WWA, Marin AV, Regueiro JR.
Front Immunol. 2022 Sep 2;13:978658. - Phenotypic and functional analysis of γδ T cells in the pathogenesis of human T-cell lymphotropic virus type 1 infection.
Ruggieri M, Ducasa N, Juraske C, Polo VG, Berini C, Quiroga MF, Christopoulos P, Minguet S, Biglione M, Schamel WW.
Front Immunol. 2022 Aug 11;13:920888. - Hyaluronic acid-GPRC5C signalling promotes dormancy in haematopoietic stem cells.
Zhang YW, Mess J, Aizarani N, Mishra P, Johnson C, Romero-Mulero MC, Rettkowski J, Schönberger K, Obier N, Jäcklein K, Woessner NM, Lalioti ME, Velasco-Hernandez T, Sikora K, Wäsch R, Lehnertz B, Sauvageau G, Manke T, Menendez P, Walter SG, Minguet S, Laurenti E, Günther S, Grün D, Cabezas-Wallscheid N.
Nat Cell Biol. 2022 Jul;24(7):1038-1048. - A set point in the selection of the αβTCR T cell repertoire imposed by pre-TCR signaling strength.
Bovolenta ER, García-Cuesta EM, Horndler L, Ponomarenko J, Schamel WW, Mellado M, Castro M, Abia D, van Santen HM.
Proc Natl Acad Sci U S A. 2022 May 31;119(22):e2201907119 - Inborn errors of immunity and immunodeficiencies: Antibody-mediated pathology and autoimmunity as a consequence of impaired immune reactions.
Minguet S, Nyström A, Kiritsi D, Rizzi M.
Eur J Immunol. 2022 Apr 20.Online ahead of print.
2021
- Cholesterol binds in a reversed orientation to TCRβ-TM in which its OH group is localized to the center of the lipid bilayer
Wu H, Cao R, Wei S, Pathan-Chhatbar S, Wen M, Wu B, Schamel W, Wang S, OuYang B
J Mol Biol. 2021 Dec 03;433(24):0022-2836 - Anesthesia triggers drug delivery to experimental glioma in mice by hijacking caveolar transport
Spieth L, Berghoff SA, Stumpf SK, Winchenbach J, Michaelis T, Watanabe T, Gerndt N, Düking T, Hofer S, Ruhwedel T, Shaib AH, Willig K, Kronenberg K, Karst U, Frahm J, Rhee JS, Minguet S, Möbius W, Kruse N, von der Brelie C, Michels P, Stadelmann C, Hülper P, Saher G
Neurooncol Adv. 2021 Sep 20;3(1) - Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-associated viral vectors
Hörner M, Jerez-Longres C, Hudek A, Hook S, Yousefi OS, Schamel WWA, Hörner C, Zurbriggen MD, Ye H, Wagner HJ, Weber W
Sci Adv. 2021 Jun 16;7(25):eabf0797 - Wiskott-Aldrich Syndrome Protein: Roles in Signal Transduction in T Cells
Ngoenkam J, Paensuwan P, Wipa P, Schamel WWA and Pongcharoen S.
Cell Dev. Biol. 2021 Jun 08;9:674572 - Cross-TCR Antagonism Revealed by Optogenetically Tuning the Half-Life of the TCR Ligand Binding
Yousefi OS, Ruggieri M, Idstein V, von Prillwitz KU, Herr LA, Chalupsky J, Köhn M, Weber W, Timmer J, Schamel WWA.
Int. J. Mol. Sci. 2021 May 06;22(9):4920. - Low Pre-Transplant Caveolin-1 Serum Concentrations Are Associated with Acute Cellular Tubulointerstitial Rejection in Kidney Transplantation
Emmerich F, Zschiedrich S, Reichenbach-Braun C, Süsal C, Minguet S, Pauly MC, Seidl M
Molecules. 2021 Apr 30;26(9):2648 - Cooperative Interaction of Nck and Lck Orchestrates Optimal TCR Signaling
Hartl FA, Ngoenkam J, Beck-Garcia E, Cerqueira L, Wipa P, Paensuwan P, Suriyaphol P, Mishra P, Schraven B, Günther S, Pongcharoen S, Schamel WWA, Minguet S
Cells 2021 Apr 07;10(4):834. - The role of cholesterol in TCR signalling in autoimmune diseases
Pathan-Chhatbar S, Chevalier N, Voll RE, Schamel WW
Medical Research Archives 2021 Feb 25;9(2):2375-1924. - NLRC5 promotes transcription of BTN3A1-3 genes and Vγ9Vδ2 T cell-mediated killing
Dang AT, Strietz J, Zenobi A, Khameneh HJ, Brandl SM, Lozza L, Conradt G, Kaufmann SHE, Reith W, Kwee I, Minguet S, Chelbi ST, Guarda G
iScience 2021 Jan 22;24(1):101900 - Direct Regulation of the T Cell Antigen Receptor’s Activity by Cholesterol
Pathan-Chhatbar S, Drechsler C, Richter K, Morath A, Wu W, OuYang B, Xu C, Schamel WW
Front Cell Dev Biol. 2021 Jan 08
2020
- Caveolin-1, tetraspanin CD81 and flotillins in lymphocyte cell membrane organization, signaling and immunopathology
Schaffer A, Minguet S
Biochem Soc Trans. 2020 Nov 26;48(6):2387–2397. - Tyrosine 192 within the SH2 domain of the Src-protein tyrosine kinase p56Lck regulates T-cell activation independently of Lck/CD45 interactions
Kästle M, Merten C, Hartig R, Kaehne T, Liaunardy-Jopeace A, Woessner NM, Schamel WW, James J, Minguet S, Simeoni L, Schraven B
Cell Com and Sig. 2020 Nov 23;18(183). - Boswellia carteri extract and 3-O-acetyl-alpha-boswellic acid suppress T cell function
Zimmermann-Klemda AM, Reinhardt JK, Nilsuc T, Morath A, Falangaa CM, Schamel WW, Huber R, Hamburger M, Gründemann C
Fitoterapia. 2020 Okt;146:104694. - PSMA-Directed CAR T Cells Combined with Low-Dose Docetaxel Treatment Induce Tumor Regression in a Prostate Cancer Xenograft Model
Alzubi J, Dettmer-Monaco V, Kuehle J, Thorausch N, Seidl M, Taromi S, Schamel W, Zeiser R, Abken H, Cathomen T, Wolf P
Mol Ther Oncolytics. 2020 Sep 25;18:226-235. - Noncanonical binding of Lck to CD3ε promotes TCR signaling and CAR function
Hartl FA, Beck-Garcìa E, Woessner NM, Flachsmann LJ, Velasco Cárdenas RMH, Brandl SM, Taromi S, Fiala GJ, Morath A, Mishra P, Yousefi OS, Zimmermann J, Hoefflin N, Köhn M, Wöhrl BM, Zeiser R, Schweimer K, Günther S, Schamel WW, Minguet S
Nat Immunol. 2020 Jul 20;21:902–913. - CCR5 deficiency impairs CD4+ T-cell memory responses and antigenic sensitivity through increased ceramide synthesis.
Martín-Leal A, Blanco R, Casas J, Sáez ME, Rodríguez-Bovolenta E, de Rojas I, Drechsler C, Real LM, Fabrias G, Ruíz A, Castro M, Schamel WW, Alarcón B, van Santen HM, Mañes S.
EMBO J. 2020 Jun 11:e104749. - Immunosuppressive Activity of Artemisia argyi Extract and Isolated Compounds.
Zimmermann-Klemd AM, Reinhardt JK, Morath A, Schamel WW, Steinberger P, Leitner J, Huber R, Hamburger M, Gründemann C.
Front Pharmacol. 2020 Apr 8;11:402. - Production, Purification and Characterization of Recombinant Biotinylated Phytochrome B for Extracellular Optogenetics
HörnerM, Yousefi OS, Schamel WW, and Weber W
Bio-protocol. 2020 Mar 05;10(5):e3541. - αβ and γδ T cell receptors: Similar but different.
Morath A, Schamel WW.
J Leukoc Biol. 2020 Jun;107(6):1045-1055. - Implications of T cell receptor biology on the development of new T cell therapies for cancer.
Hardy IR, Schamel WW, Baeuerle PA, Getts DR, Hofmeister R.
Immunotherapy. 2020 Jan;12(1):89-103. - Optogenetic Tuning of Ligand Binding to The Human T cell Receptor Using The opto-ligand-TCR System
Yousefi OS, Hörner M, Wess M, Idstein V, Weber W, Schamel WWA
Bio-protocol. 2020 Mar 05;10(5):e3540. - Actin polymerization regulates recruitment of Nck to CD3ε upon T-cell receptor triggering.
Wipa P, Paensuwan P, Ngoenkam J, Woessner NM, Minguet S, Schamel WW, Pongcharoen S.
Immunology. 2020 Mar;159(3):298-308. - Molecular mechanism of SHP2 activation by PD-1 stimulation
Marasco M, Berteotti A, Weyershaeuser J, Thorausch N, Sikorska J, Krausze J, Brandt HJ, Kirkpatrick J, Rios P, Schamel WW, Köhn M*,Carlomagno T*
Sci Adv. 2020 Jan 31;6(5):eaay4458.
2019
- Rational design of a trimeric APRIL-based CAR-binding domain enables efficient targeting of multiple myeloma.
Schmidts A, Ormhøj M, Choi BD, Taylor AO, Bouffard AA, Scarfò I, Larson RC, Frigault MJ, Gallagher K, Castano AP, Riley LS, Cabral ML, Boroughs AC, Velasco Cárdenas RM, Schamel W, Zhou J, Mackay S, Tai YT, Anderson KC, Maus MV.
Blood Adv. 2019 Nov 12;3(21):3248-3260. - Split intein-mediated selection of cells containing two plasmids using a single antibiotic.
Palanisamy N, Degen A, Morath A, Ballestin Ballestin J, Juraske C, Öztürk MA, Sprenger GA, Youn JW, Schamel WW, Di Ventura B.
Nat Commun. 2019 Oct 31;10(1):4967. - The TCR is an allosterically regulated macromolecular machinery changing its conformation while working.
Schamel WW, Alarcon B, Minguet S.
Immunol Rev. 2019 Sep;291(1):8-25. - Proximal Lck Promoter-Driven Cre Function Is Limited in Neonatal and Ineffective in Adult γδ T Cell Development.
Fiala GJ, Schaffer AM, Merches K, Morath A, Swann J, Herr LA, Hils M, Esser C, Minguet S, Schamel WWA.
J Immunol. 2019 Jul 15;203(2):569-579. - Synthetic TRuC receptors engaging the complete T cell receptor for potent anti-tumor response.
Baeuerle PA, Ding J, Patel E, Thorausch N, Horton H, Gierut J, Scarfo I, Choudhary R, Kiner O, Krishnamurthy J, Le B, Morath A, Baldeviano GC, Quinn J, Tavares P, Wei Q, Weiler S, Maus MV, Getts D, Schamel WW, Hofmeister R.
Nat Commun. 2019 May 7;10(1):2087. - Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor.
Yousefi OS, Günther M, Hörner M, Chalupsky J, Wess M, Brandl SM, Smith RW, Fleck C, Kunkel T, Zurbriggen MD, Höfer T, Weber W, Schamel WW.
Elife. 2019 Apr 5;8. pii: e4247 - Light-controlled affinity purification of protein complexes exemplified by the resting ZAP70 interactome.
Hörner M, Eble J, Yousefi OS, Schwarz J, Warscheid B, Weber W, Schamel WWA.
Front Immunol. 2019 Feb 26;10:226. - Phytochrome-based extracellular matrix with reversibly tunable mechanical properties.
Hörner M, Raute K, Hummel B, Madl J, Creusen G, Thomas OS, Christen EH, Hotz N, Gübeli RJ, Engesser R, Rebmann B, Lauer J, Rolauffs B, Timmer J, Schamel WWA, Pruszak J, Römer W, Zurbriggen MD, Friedrich C, Walther A, Minguet S, Sawarkar R, Weber W.
Adv Mater. 2019 Mar;31(12):e1806727. - Optogenetic control of integrin-matrix interaction.
Baaske J, Mühlhäuser WWD, Yousefi OS, Zanner S, Radziwill G, Hörner M, Schamel WWA, Weber W.
Commun Biol. 2019 Jan 8;2:15.
2018
- Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells.
Baaske J, Gonschorek P, Engesser R, Dominguez-Monedero A, Raute K, Fischbach P, Müller K, Cachat E, Schamel WWA, Minguet S, Davies JA, Timmer J, Weber W, Zurbriggen MD.
Sci Rep. 2018 Oct 9;8(1):15024. - Anti-CD3 Fab Fragments Enhance Tumor Killing by Human γδ T Cells Independent of Nck Recruitment to the γδ T Cell Antigen Receptor.
Juraske C, Wipa P, Morath A, Hidalgo JV, Hartl FA, Raute K, Oberg HH, Wesch D, Fisch P, Minguet S, Pongcharoen S, Schamel WW.
Front Immunol. 2018 Jul 9;9:1579. - A window of opportunity for cooperativity in the T Cell Receptor.
Martin-Blanco N, Blanco R, Alda-Catalinas C, Bovolenta ER, Oeste CL, Palmer E, Schamel WW, Lythe G, Molina-París C, Castro M, Alarcon B.
Nat Commun. 2018 Jul 5;9(1):2618. - Oncogenic JAK2V617F causes PD-L1 expression, mediating immune escape in myeloproliferative neoplasms.
Prestipino A, Emhardt AJ, Aumann K, O’Sullivan D, Gorantla SP, Duquesne S, Melchinger W, Braun L, Vuckovic S, Boerries M, Busch H, Halbach S, Pennisi S, Poggio T, Apostolova P, Veratti P, Hettich M, Niedermann G, Bartholomä M, Shoumariyeh K, Jutzi JS, Wehrle J, Dierks C, Becker H, Schmitt-Graeff A, Follo M, Pfeifer D, Rohr J, Fuchs S, Ehl S, Hartl FA, Minguet S, Miething C, Heidel FH, Kröger N, Triviai I, Brummer T, Finke J, Illert AL, Ruggiero E, Bonini C, Duyster J, Pahl HL, Lane SW, Hill GR, Blazar BR, von Bubnoff N, Pearce EL, Zeiser R.
Sci Transl Med. 2018 Feb 21;10(429). - Caveolin-1: The unnoticed player in TCR and BCR signaling.
Fiala GJ, Minguet S.
Adv Immunol. 2018;137:83-133. - Selected signalling proteins recruited to the T-cell receptor-CD3 complex.
Ngoenkam J, Schamel WW, Pongcharoen S.
Immunology. 2018 Jan;153(1):42-50.
2017
- Regulatory T cells characterized by low Id3 expression are highly suppressive and accumulate during chronic infection.
Rauch KS, Hils M, Menner AJ, Sigvardsson M, Minguet S, Aichele P, Schachtrup C, Schachtrup K.
Oncotarget. 2017 Oct 27;8(61):102835-102851. - Caveolin-1-dependent nanoscale organization of the BCR regulates B cell tolerance.
Minguet S, Kläsener K, Schaffer AM, Fiala GJ, Osteso-Ibánez T, Raute K, Navarro-Lérida I, Hartl FA, Seidl M, Reth M, Del Pozo MA.
Nat Immunol. 2017 Oct;18(10):1150-1159. - The BTG2-PRMT1 module limits pre-B cell expansion by regulating the CDK4-Cyclin-D3 complex.
Dolezal E, Infantino S, Drepper F, Börsig T, Singh A, Wossning T, Fiala GJ, Minguet S, Warscheid B, Tarlinton DM, Jumaa H, Medgyesi D, Reth M.
Nat Immunol. 2017 Aug;18(8):911-920. - Biglycan expression in the melanoma microenvironment promotes invasiveness via increased tissue stiffness inducing integrin-β1 expression.
Andrlová H, Mastroianni J, Madl J, Kern JS, Melchinger W, Dierbach H, Wernet F, Follo M, Technau-Hafsi K, Has C, Rao Mittapalli V, Idzko M, Herr R, Brummer T, Ungefroren H, Busch H, Boerries M, Narr A, Ihorst G, Vennin C, Schmitt-Graeff A, Minguet S, Timpson P, Duyster J, Meiss F, Römer W, Zeiser R.
Oncotarget. 2017 Jun 27;8(26):42901-42916 - Key role of the scavenger receptor MARCO in mediating adenovirus infection and subsequent innate responses of macrophages.
Maler MD, Nielsen PJ, Stichling N, Cohen I, Ruzsics Z, Wood C, Engelhard P, Suomalainen M, Gyory I, Huber M, Müller-Quernheim J, Schamel WWA, Gordon S, Jakob T, Martin SF, Jahnen-Dechent W, Greber UF, Freudenberg MA, Fejer G.
MBio. 2017 Aug 1;8(4). - The Allostery Model of TCR Regulation.
Schamel WW, Alarcon B, Höfer T, Minguet S.
J Immunol. 2017 Jan 1;198(1):47-52. Review.
2016
- Id3 Maintains Foxp3 Expression in Regulatory T Cells by Controlling a Transcriptional Network of E47, Spi-B, and SOCS3.
Rauch KS, Hils M, Lupar E, Minguet S, Sigvardsson M, Rottenberg ME, Izcue A, Schachtrup C, Schachtrup K.
Cell Rep. 2016 Dec 13;17(11):2827-2836. - Early onset combined immunodeficiency and autoimmunity in patients with loss-of-function mutation in LAT.
Keller B, Zaidman I, Yousefi OS, Hershkovitz D, Stein J, Unger S, Schachtrup K, Sigvardsson M, Kuperman AA, Shaag A, Schamel WW, Elpeleg O, Warnatz K, Stepensky P.
J Exp Med. 2016 Jun 27;213(7):1185-99. - Caveolin-1 regulates TCR signal strength and regulatory T-cell differentiation into alloreactive T cells.
Schönle A, Hartl FA, Mentzel J, Nöltner T, Rauch KS, Prestipino A, Wohlfeil SA, Apostolova P, Hechinger AK, Melchinger W, Fehrenbach K, Guadamillas MC, Follo M, Prinz G, Ruess AK, Pfeifer D, Angel Del Pozo M, Schmitt-Graeff A, Duyster J, Hippen KI, Blazar BR, Schachtrup K, Minguet S, Zeiser R.
Blood. 2016 Apr 14;127(15):1930-9 - Inhibition of T cell receptor signaling by cholesterol sulfate, a naturally occurring derivative of membrane cholesterol.
Wang F, Beck-García K, Zorzin C, Schamel WW, Davis MM.
Nat Immunol. 2016 Jul;17(7):844-50. - A Cholesterol-Based Allostery Model of T Cell Receptor Phosphorylation.
Swamy M, Beck-Garcia K, Beck-Garcia E, Hartl FA, Morath A, Yousefi OS, Dopfer EP, Molnár E, Schulze AK, Blanco R, Borroto A, Martín-Blanco N, Alarcon B, Höfer T, Minguet S, Schamel WW.
Immunity. 2016 May 17;44(5):1091-101. - Activation loop phosphorylation regulates B-Raf in vivo and transformation by B-Raf mutants.
Köhler M, Röring M, Schorch B, Heilmann K, Stickel N, Fiala GJ, Schmitt LC, Braun S, Ehrenfeld S, Uhl FM, Kaltenbacher T, Weinberg F, Herzog S, Zeiser R, Schamel WW, Jumaa H, Brummer T.
EMBO J. 2016 Jan 18;35(2):143-61 - Nck Binds to the T Cell Antigen Receptor Using Its SH3.1 and SH2 Domains in a Cooperative Manner, Promoting TCR Functioning.
Paensuwan P, Hartl FA, Yousefi OS, Ngoenkam J, Wipa P, Beck-Garcia E, Dopfer EP, Khamsri B, Sanguansermsri D, Minguet S, Schamel WW, Pongcharoen S.
J Immunol. 2016 Jan 1;196(1):448-58.
2015
- Caveolin-1 deficiency induces a MEK-ERK1/2-Snail-1-dependent epithelial-mesenchymal transition and fibrosis during peritoneal dialysis.
Strippoli R, Loureiro J, Moreno V, Benedicto I, Lozano ML, Barreiro O, Pellinen T, Minguet S, Foronda M, Osteso MT, Calvo E, Vázquez J, Cabrera ML, Del Pozo MA.
EMBO Mol Med. 2015 Mar 4;7(3):357 - Response to Comment on “A Novel Thymoma-Associated Immunodeficiency with Increased Naive T Cells and Reduced CD247 Expression”.
Fisch P, Christopoulos P, Marx A, Schamel WW.
J Immunol. 2015 Oct 15;195(8):3505-6. - Kidins220/ARMS binds to the B cell antigen receptor and regulates B cell development and activation.
Fiala GJ, Janowska I, Prutek F, Hobeika E, Satapathy A, Sprenger A, Plum T, Seidl M, Dengjel J, Reth M, Cesca F, Brummer T, Minguet S, Schamel WW.
J Exp Med. 2015 Sep 21;212(10):1693-708. - The ubiquitin-specific protease USP8 is critical for the development and homeostasis of T cells.
Dufner A, Kisser A, Niendorf S, Basters A, Reissig S, Schönle A, Aichem A, Kurz T, Schlosser A, Yablonski D, Groettrup M, Buch T, Waisman A, Schamel WW, Prinz M, Knobeloch KP.
Nat Immunol. 2015 Sep;16(9):950-60. - SYK expression endows human ZAP70-deficient CD8 T cells with residual TCR signaling.
Hauck F, Blumenthal B, Fuchs S, Lenoir C, Martin E, Speckmann C, Vraetz T, Mannhardt-Laakmann W, Lambert N, Gil M, Borte S, Audrain M, Schwarz K, Lim A, Schamel WW, Fischer A, Ehl S, Rensing-Ehl A, Picard C, Latour S.
Clin Immunol. 2015 Jul 14;161(2):103-109.
- Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish.
Beyer HM, Juillot S, Herbst K, Samodelov SL, Müller K, Schamel WW, Römer W, Schäfer E, Nagy F, Strähle U, Weber W, Zurbriggen MD.
ACS Synth Biol. 2015 Sep 18;4(9):951-8. - A novel thymoma-associated immunodeficiency with increased naive T cells and reduced CD247 expression.
Christopoulos P, Dopfer EP, Malkovsky M, Esser PR, Schaefer HE, Marx A, Kock S, Rupp N, Lorenz MR, Schwarz K, Harder J, Martin SF, Werner M, Bogdan C, Schamel WW, Fisch P.
J Immunol. 2015 Apr 1;194(7):3045-53. - Preface to special issue on nanoscale membrane organisations.
Alarcón B, Reth M, Schamel W.
Biochim Biophys Acta. 2015 Apr;1853(4):765-6. - T Cell Expansion Is the Limiting Factor of Virus Control in Mice with Attenuated TCR Signaling: Implications for Human Immunodeficiency.
Hillen KM, Gather R, Enders A, Pircher H, Aichele P, Fisch P, Blumenthal B, Schamel WW, Straub T, Goodnow CC, Ehl S.
J Immunol. 2015 Mar 15;194(6):2725-34. - Nanoclusters of the resting T cell antigen receptor (TCR) localize to non-raft domains.
Beck-García K, Beck-García E, Bohler S, Zorzin C, Sezgin E, Levental I, Alarcón B, Schamel WW.
Biochim Biophys Acta. 2015 Apr;1853(4):802-9.
2014
- Caveolin-1 deficiency induces a MEK-ERK1/2-Snail-1-dependent epithelial-mesenchymal transition and fibrosis during peritoneal dialysis.
Strippoli R, Loureiro J, Moreno V, Benedicto I, Pérez Lozano ML, Barreiro O, Pellinen T, Minguet S, Foronda M, Osteso MT, Calvo E, Vázquez J, López Cabrera M, del Pozo MA.
EMBO Mol Med. 2014 Dec 30;7(1):102-23 - Conformational changes in the T cell receptor differentially determine T cell subset development in mice.
Blanco R, Borroto A, Schamel W, Pereira P, Alarcon B.
Sci Signal. 2014 Dec 2;7(354):ra115. - Non-overlapping functions of Nck1 and Nck2 adaptor proteins in T cell activation.
Ngoenkam J, Paensuwan P, Preechanukul K, Khamsri B, Yiemwattana I, Beck-García E, Minguet S, Schamel WW, Pongcharoen S.
Cell Commun Signal. 2014 Mar 26;12:21
- The CD3 Conformational Change in the ?? T Cell Receptor Is Not Triggered by Antigens but Can Be Enforced to Enhance Tumor Killing.
Dopfer EP, Hartl FA, Oberg HH, Siegers GM, Yousefi OS, Kock S, Fiala GJ, Garcillán B, Sandstrom A, Alarcón B, Regueiro JR, Kabelitz D, Adams EJ, Minguet S, Wesch D, Fisch P, Schamel WW.
Cell Rep. 2014 Jun 12;7(5):1704-15. - Relevance of Nck-CD3{varepsilon} Interaction for T Cell Activation In Vivo.
Borroto A, Arellano I, Blanco R, Fuentes M, Orfao A, Dopfer EP, Prouza M, Suchànek M, Schamel WW, Alarcón B.
J Immunol. 2014 Mar 1;192(5):2042-53 - A common single nucleotide polymorphism impairs B-cell activating factor receptor’s multimerization, contributing to common variable immunodeficiency.
Pieper K, Rizzi M, Speletas M, Smulski CR, Sic H, Kraus H, Salzer U, Fiala GJ, Schamel WW, Lougaris V, Plebani A, Hammarstrom L, Recher M, Germenis AE, Grimbacher B, Warnatz K, Rolink AG, Schneider P, Notarangelo LD, Eibel H.
J Allergy Clin Immunol. 2014 Apr;133(4):1222-5 - Activation of the TCR complex by small chemical compounds.
Louis-Dit-Sully C, Schamel WW.
EXS. 2014;104:25-39. - Activation of the TCR complex by peptide-MHC and superantigens.
Louis-Dit-Sully C, Blumenthal B, Duchniewicz M, Beck-Garcia K, Fiala GJ, Beck-García E, Mukenhirn M, Minguet S, Schamel WW.
EXS. 2014;104:9-23.
2013
- Pre-clustering of the B cell antigen receptor demonstrated by mathematically extended electron microscopy.
Fiala GJ, Kaschek D, Blumenthal B, Reth M, Timmer J, Schamel WW.
Front Immunol. 2013 Dec 6;4:427 - Visualization of TCR nanoclusters via immunogold labeling, freeze-etching, and surface replication.
Fiala GJ, Rejas MT, Schamel WW, van Santen HM.
Methods Cell Biol. 2013;117:391-410.
- Analysis of interactions between proteins and fatty acids or cholesterol using a fatty acid/cholesterol pull-down assay.
Beck-García E, Beck-García K, Schlosser A, Schamel WW.
Anal Biochem. 2013 May 15;436(2):75-7 - Kidins220/ARMS associates with B-Raf and the TCR, promoting sustained Erk signaling in T cells.
Deswal S, Meyer A, Fiala GJ, Eisenhardt AE, Schmitt LC, Salek M, Brummer T, Acuto O, Schamel WW.
J Immunol. 2013 Mar 1;190(5):1927-35 - Organization of the resting TCR in nanoscale oligomers.
Schamel WW, Alarcón B.
Immunol Rev. 2013 Jan;251(1):13-20. - Semi-automatic determination of cell surface areas used in systems biology.
Morath V, Keuper M, Rodriguez-Franco M, Deswal S, Fiala G, Blumenthal B, Kaschek D, Timmer J, Neuhaus G, Ehl S, Ronneberger O, Schamel WW.
Front Biosci (Elite Ed). 2013 Jan 1;5:533-45. - Nck recruitment to the TCR required for ZAP70 activation during thymic development.
Borroto A, Arellano I, Dopfer EP, Prouza M, Suchànek M, Fuentes M, Orfao A, Schamel WW, Alarcón B.
J Immunol. 2013 Feb 1;190(3):1103-12. - Antitumor activities of PSMA×CD3 diabodies by redirected T-cell lysis of prostate cancer cells.
Baum V, Bühler P, Gierschner D, Herchenbach D, Fiala GJ, Schamel WW, Wolf P, Elsässer-Beile U.
Immunotherapy. 2013 Jan;5(1):27-38
2012
- γδ T-cell conference 2012: close encounters for the fifth time.
Silva-Santos B, Schamel WW, Fisch P, Eberl M.
Eur J Immunol. 2012 Dec;42(12):3101-5. - Cholesterol and sphingomyelin drive ligand-independent T-cell antigen receptor nanoclustering.
Molnár E, Swamy M, Holzer M, Beck-García K, Worch R, Thiele C, Guigas G, Boye K, Luescher IF, Schwille P, Schubert R, Schamel WW.
J Biol Chem. 2012 Dec 14;287(51):42664-74. - Meeting report: Signal transduction meets systems biology.
Louis-Dit-Sully C, Kubatzky KF, Lindquist JA, Blattner C, Janssen O, Schamel WW.
Cell Commun Signal. 2012 Apr 30;10(1):11 - Distinct requirement for an intact dimer interface in wild-type, V600E and kinase-dead B-Raf signalling.
Röring M, Herr R, Fiala GJ, Heilmann K, Braun S, Eisenhardt AE, Halbach S, Capper D, von Deimling A, Schamel WW, Saunders DN, Brummer T.
EMBO J. 2012 May 30;31(11):2629-47. - Synthetic immune signaling.
Schamel WW, Reth M.
Curr Opin Biotechnol. 2012 Oct;23(5):780-4. - Antiviral and regulatory T cell immunity in a patient with stromal interaction molecule 1 deficiency.
Fuchs S, Rensing-Ehl A, Speckmann C, Bengsch B, Schmitt-Graeff A, Bondzio I, Maul-Pavicic A, Bass T, Vraetz T, Strahm B, Ankermann T, Benson M, Caliebe A, Fölster-Holst R, Kaiser P, Thimme R, Schamel WW, Schwarz K, Feske S, Ehl S.
J Immunol. 2012 Feb 1;188(3):1523-33.
2011
- A leaky mutation in CD3D differentially affects αβ and γδ T cells and leads to a Tαβ-Tγδ+B+NK+ human SCID.
Gil J, Busto EM, Garcillán B, Chean C, García-Rodríguez MC, Díaz-Alderete A, Navarro J, Reiné J, Mencía A, Gurbindo D, Beléndez C, Gordillo I, Duchniewicz M, Höhne K, García-Sánchez F, Fernández-Cruz E, López-Granados E, Schamel WW, Moreno-Pelayo MA, Recio MJ, Regueiro JR.
J Clin Invest. 2011 Oct;121(10):3872-6 - Increased sensitivity of antigen-experienced T cells through the enrichment of oligomeric T cell receptor complexes.
Kumar R, Ferez M, Swamy M, Arechaga I, Rejas MT, Valpuesta JM, Schamel WW, Alarcon B, van Santen HM.
Immunity. 2011 Sep 23;35(3):375-87. - A new vampire saga: the molecular mechanism of T cell trogocytosis.
Dopfer EP, Minguet S, Schamel WW.
Immunity. 2011 Aug 26;35(2):151-3. - Quantitative analysis of protein phosphorylations and interactions by multi-colour IP-FCM as an input for kinetic modelling of signalling networks.
Deswal S, Schulze AK, Höfer T, Schamel WW.
PLoS One. 2011;6(7):e22928. - Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis.
Goetz JG, Minguet S, Navarro-Lérida I, Lazcano JJ, Samaniego R, Calvo E, Tello M, Osteso-Ibáñez T, Pellinen T, Echarri A, Cerezo A, Klein-Szanto AJ, Garcia R, Keely PJ, Sánchez-Mateos P, Cukierman E, Del Pozo MA.
Cell. 2011 Jul 8;146(1):148-63. - Blue native polyacrylamide gel electrophoresis (BN-PAGE) for analysis of multiprotein complexes from cellular lysates.
Fiala GJ, Schamel WW, Blumenthal B.
J Vis Exp. 2011 Feb 24;(48). - B cell antigen receptor-induced activation of an IRAK4-dependent signaling pathway revealed by a MALT1-IRAK4 double knockout mouse model.
Dufner A, Schamel WW.
Cell Commun Signal. 2011 Mar 11;9(1):6. - ORAI1-mediated calcium influx is required for human cytotoxic lymphocyte degranulation and target cell lysis.
Maul-Pavicic A, Chiang SC, Rensing-Ehl A, Jessen B, Fauriat C, Wood SM, Sjöqvist S, Hufnagel M, Schulze I, Bass T, Schamel WW, Fuchs S, Pircher H, McCarl CA, Mikoshiba K, Schwarz K, Feske S, Bryceson YT, Ehl S.
Proc Natl Acad Sci U S A. 2011 Feb 22;108(8):3324-9
2010
- Structural characterization of the TCR complex by electron microscopy.
Arechaga I, Swamy M, Abia D, Schamel WA, Alarcón B, Valpuesta JM.
Int Immunol. 2010 Nov;22(11):897-903 - Pre-clustered TCR complexes.
Molnár E, Deswal S, Schamel WW.
FEBS Lett. 2010 Dec 15;584(24):4832-7 - Innate, antigen-independent role for T cells in the activation of the immune system by Propionibacterium acnes.
Tchaptchet S, Kirberg J, Freudenberg N, Schamel WW, Galanos C, Freudenberg MA.
Eur J Immunol. 2010 Sep;40(9):2506-16. - Stoichiometry and intracellular fate of TRIM-containing TCR complexes.
Swamy M, Siegers GM, Fiala GJ, Molnar E, Dopfer EP, Fisch P, Schraven B, Schamel WW.
Cell Commun Signal. 2010 Mar 18;8:5 - Low-valency, but not monovalent, antigens trigger the B-cell antigen receptor (BCR).
Minguet S, Dopfer EP, Schamel WW.
Int Immunol. 2010 Mar;22(3):205-12. - Detection of phosphorylated T and B cell antigen receptor species by Phos-tag SDS- and Blue Native-PAGE.
Deswal S, Beck-García K, Blumenthal B, Dopfer EP, Schamel WW.
Immunol Lett. 2010 May 4;130(1-2):51-6. - Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling.
Dopfer EP, Schöpf B, Louis-Dit-Sully C, Dengler E, Höhne K, Klescová A, Prouza M, Suchanek M, Reth M, Schamel WW.
Immunol Lett. 2010 May 4;130(1-2):43-50
2009
- Models of antigen receptor activation in the design of vaccines.
Molnár E, Dopfer EP, Deswal S, Schamel WW.
Curr Pharm Des. 2009;15(28):3237-48. - Target-dependent T-cell activation by coligation with a PSMA x CD3 diabody induces lysis of prostate cancer cells.
Bühler P, Molnar E, Dopfer EP, Wolf P, Gierschner D, Wetterauer U, Schamel WW, Elsässer-Beile U.
J Immunother. 2009 Jul-Aug;32(6):565-73. - Detection of protein complex interactions via a Blue Native-PAGE retardation assay.
Swamy M, Molnar E, Bock T, Bausch-Fluck D, Wollscheid B, Schamel WW.
Anal Biochem. 2009 Sep 15;392(2):177-9. - Purification of the T cell antigen receptor and analysis by blue-native PAGE.
Swamy M, Schamel WW.
Methods Mol Biol. 2009;514:135-50
2008
- Permissive geometry model.
Minguet S, Schamel WW.
Adv Exp Med Biol. 2008;640:113-20. - Segregation models.
Dopfer EP, Swamy M, Siegers GM, Molnar E, Yang J, Schamel WW.
Adv Exp Med Biol. 2008;640:74-81. - Clustering models.
Schamel WW, Reth M.
Adv Exp Med Biol. 2008;640:64-73.
- Interaction of KLRG1 with E-cadherin: new functional and structural insights.
Rosshart S, Hofmann M, Schweier O, Pfaff AK, Yoshimoto K, Takeuchi T, Molnar E, Schamel WW, Pircher H.
Eur J Immunol. 2008 Dec;38(12):3354-64. - Enhanced B-cell activation mediated by TLR4 and BCR crosstalk.
Minguet S, Dopfer EP, Pollmer C, Freudenberg MA, Galanos C, Reth M, Huber M, Schamel WW.
Eur J Immunol. 2008 Sep;38(9):2475-87 - Differential antibody binding to the surface alphabetaTCR.CD3 complex of CD4+ and CD8+ T lymphocytes is conserved in mammals and associated with differential glycosylation.
Rossi NE, Reiné J, Pineda-Lezamit M, Pulgar M, Meza NW, Swamy M, Risueno R, Schamel WW, Bonay P, Fernández-Malavé E, Regueiro JR.
Int Immunol. 2008 Oct;20(10):1247-58. - Two-dimensional blue native polyacrylamide gel electrophoresis.
Schamel WW.
Curr Protoc Cell Biol. 2008 Mar;Chapter 6:Unit 6.10. - T cell receptor engagement triggers its CD3epsilon and CD3zeta subunits to adopt a compact, locked conformation.
Risueño RM, Schamel WW, Alarcón B.
PLoS One. 2008 Mar 5;3(3):e1747 - The 450 kDa TCR Complex has a Stoichiometry of alphabetagammaepsilondeltaepsilonzetazeta.
Swamy M, Dopfer EP, Molnar E, Alarcón B, Schamel WW.
Scand J Immunol. 2008 Apr;67(4):418-20 - The extracellular part of zeta is buried in the T cell antigen receptor complex.
Minguet S, Swamy M, Dopfer EP, Dengler E, Alarcón B, Schamel WW.
Immunol Lett. 2008 Mar 15;116(2):203-10 - The short length of the extracellular domain of zeta is crucial for T cell antigen receptor function.
Minguet S, Swamy M, Schamel WW.
Immunol Lett. 2008 Mar 15;116(2):195-202.
- A permissive geometry model for TCR-CD3 activation.
Minguet S, Schamel WW.
Trends Biochem Sci. 2008 Feb;33(2):51-7. - A bispecific diabody directed against prostate-specific membrane antigen and CD3 induces T-cell mediated lysis of prostate cancer cells.
Bühler P, Wolf P, Gierschner D, Schaber I, Katzenwadel A, Schultze-Seemann W, Wetterauer U, Tacke M, Swamy M, Schamel WW, Elsässer-Beile U.
Cancer Immunol Immunother. 2008 Jan;57(1):43-52.
2007
- The TCR binding site does move.
Schamel WW, Reth M.
Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16398-9 - Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.
Siegers GM, Swamy M, Fernández-Malavé E, Minguet S, Rathmann S, Guardo AC, Pérez-Flores V, Regueiro JR, Alarcón B, Fisch P, Schamel WW.
J Exp Med. 2007 Oct 29;204(11):2537-44. - A native antibody-based mobility-shift technique (NAMOS-assay) to determine the stoichiometry of multiprotein complexes.
Swamy M, Minguet S, Siegers GM, Alarcón B, Schamel WW.
J Immunol Methods. 2007 Jul 31;324(1-2):74-83. - Association of protein kinase C-delta with the B cell antigen receptor complex.
Pracht C, Minguet S, Leitges M, Reth M, Huber M.
Cell Signal. 2007 Apr;19(4):715-22.
- Oxysterol-binding protein-related protein (ORP) 9 is a PDK-2 substrate and regulates Akt phosphorylation.
Lessmann E, Ngo M, Leitges M, Minguet S, Ridgway ND, Huber M.
Cell Signal. 2007 Feb;19(2):384-92. - High-sensitivity detection and quantitative analysis of native protein-protein interactions and multiprotein complexes by flow cytometry.
Schrum AG, Gil D, Dopfer EP, Wiest DL, Turka LA, Schamel WW, Palmer E.
Sci STKE. 2007 Jun 5;2007(389):pl2. - Full activation of the T cell receptor requires both clustering and conformational changes at CD3.
Minguet S, Swamy M, Alarcón B, Luescher IF, Schamel WW.
Immunity. 2007 Jan;26(1):43-54.
2006
- Overlapping functions of human CD3delta and mouse CD3gamma in alphabeta T-cell development revealed in a humanized CD3gamma-mouse.
Fernández-Malavé E, Wang N, Pulgar M, Schamel WW, Alarcón B, Terhorst C.
Blood. 2006 Nov 15;108(10):3420-7. - Identification of disulfide bonds in the Ig-alpha/Ig-beta component of the B cell antigen receptor using the Drosophila S2 cell reconstitution system.
Siegers GM, Yang J, Duerr CU, Nielsen PJ, Reth M, Schamel WW.
Int Immunol. 2006 Sep;18(9):1385-96. - Blue native polyacrylamide gel electrophoresis (BN-PAGE) for the identification and analysis of multiprotein complexes.
Swamy M, Siegers GM, Minguet S, Wollscheid B, Schamel WW.
Sci STKE. 2006 Jul 25;2006(345):pl4.
- T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity.
Alarcón B, Swamy M, van Santen HM, Schamel WW.
EMBO Rep. 2006 May;7(5):490-5 - A conformation- and avidity-based proofreading mechanism for the TCR-CD3 complex.
Schamel WW, Risueño RM, Minguet S, Ortíz AR, Alarcón B.
Trends Immunol. 2006 Apr;27(4):176-82 - Two dimensional Blue Native-/SDS-PAGE analysis of SLP family adaptor protein complexes.
Swamy M, Kulathu Y, Ernst S, Reth M, Schamel WW.
Immunol Lett. 2006 Apr 15;104(1-2):131-7.
2005
- Differences in pairing and cluster formation of T cell receptor alpha- and beta-chains in T cell clones and fusion hybridomas.
Hellwig S, Schamel WW, Pflugfelder U, Gerlich B, Weltzien HU.
Immunobiology. 2005;210(9):685-94. - Subproteomic analysis of metal-interacting proteins in human B cells.
Heiss K, Junkes C, Guerreiro N, Swamy M, Camacho-Carvajal MM, Schamel WW, Haidl ID, Wild D, Weltzien HU, Thierse HJ.
Proteomics. 2005 Sep;5(14):3614-22.
- Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response.
Schamel WW, Arechaga I, Risueño RM, van Santen HM, Cabezas P, Risco C, Valpuesta JM, Alarcón B.
J Exp Med. 2005 Aug 15;202(4):493-503. - Adenosine and cAMP are potent inhibitors of the NF-kappa B pathway downstream of immunoreceptors.
Minguet S, Huber M, Rosenkranz L, Schamel WW, Reth M, Brummer T.
Eur J Immunol. 2005 Jan;35(1):31-41.
2004
- Biochemical differences in the alphabeta T cell receptor.CD3 surface complex between CD8+ and CD4+ human mature T lymphocytes.
Zapata DA, Schamel WW, Torres PS, Alarcón B, Rossi NE, Navarro MN, Toribio ML, Regueiro JR.
J Biol Chem. 2004 Jun 4;279(23):24485-92. - Two-dimensional Blue native/SDS gel electrophoresis of multi-protein complexes from whole cellular lysates: a proteomics approach.
Camacho-Carvajal MM, Wollscheid B, Aebersold R, Steimle V, Schamel WW.
Mol Cell Proteomics. 2004 Feb;3(2):176-82.
2003
- A population of c-Kit(low)(CD45/TER119)- hepatic cell progenitors of 11-day postcoitus mouse embryo liver reconstitutes cell-depleted liver organoids.
Minguet S, Cortegano I, Gonzalo P, Martínez-Marin JA, de Andrés B, Salas C, Melero D, Gaspar ML, Marcos MA.
J Clin Invest. 2003 Oct;112(8):1152-63 - A high-molecular-weight complex of membrane proteins BAP29/BAP31 is involved in the retention of membrane-bound IgD in the endoplasmic reticulum.
Schamel WW, Kuppig S, Becker B, Gimborn K, Hauri HP, Reth M.
Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9861-6. - Initiation of TCR signaling: regulation within CD3 dimers.
Alarcón B, Gil D, Delgado P, Schamel WW.
Immunol Rev. 2003 Feb;191:38-46.
2002
- The first 3 days of B-cell development in the mouse embryo.
de Andrés B, Gonzalo P, Minguet S, Martínez-Marin JA, Soro PG, Marcos MA, Gaspar ML.+
Blood. 2002 Dec 1;100(12):4074-81. - Amplification of B cell antigen receptor signaling by a Syk/ITAM positive feedback loop.
Rolli V, Gallwitz M, Wossning T, Flemming A, Schamel WW, Zürn C, Reth M.
Mol Cell. 2002 Nov;10(5):1057-69. - Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation.
Gil D, Schamel WW, Montoya M, Sánchez-Madrid F, Alarcón B.
Cell. 2002 Jun 28;109(7):901-12. - Role of zeta PKC in B-cell signaling and function.
Martin P, Duran A, Minguet S, Gaspar ML, Diaz-Meco MT, Rennert P, Leitges M, Moscat J.
EMBO J. 2002 Aug 1;21(15):4049-57.
2001
- Both B and gammadelta TCR(+) lymphocytes regulate alphabeta TCR(+) lymphocytes involved in superantigen specific responses.
Izcue A, Morales G, Minguet S, Sánchez-Movilla A, Morales P, Martínez-M JA, Gaspar ML, Marcos MA.
Eur J Immunol. 2001 Sep;31(9):2811-7. - Long-lived polyclonal B-cell lines derived from midgestation mouse embryo lymphohematopoietic progenitors reconstitute adult immunodeficient mice.
Martínez-M JA, Minguet S, Gonzalo P, Soro PG, de Andrés B, Izcue A, Marcos MA, Gaspar ML.
Blood. 2001 Sep 15;98(6):1862-71. - Biotinylation of protein complexes may lead to aggregation as well as to loss of subunits as revealed by Blue Native PAGE.
Schamel WW.
J Immunol Methods. 2001 Jun 1;252(1-2):171-4.
2000
- An unsolved problem of the clonal selection theory and the model of an oligomeric B-cell antigen receptor.
Reth M, Wienands J, Schamel WW.
Immunol Rev. 2000 Aug;176:10-8 - Monomeric and oligomeric complexes of the B cell antigen receptor.
Schamel WW, Reth M.
Immunity. 2000 Jul;13(1):5-14. - Stability of the B cell antigen receptor complex.
Schamel WW, Reth M.
Mol Immunol. 2000 Apr;37(5):253-9.
1999
- Comparison of the tamoxifen regulated chimeric Cre recombinases MerCreMer and CreMer.
Verrou C, Zhang Y, Zürn C, Schamel WW, Reth M.
Biol Chem. 1999 Dec;380(12):1435-8. - Detection of proteins after biotinylation within polyacrylamide gels (BIG method).
Schamel WW.
Anal Biochem. 1999 Oct 1;274(1):144-6.
1998
- Developmental hematopoiesis.
Morales-Alcelay S, Copín SG, Martínez JA, Morales P, Minguet S, Gaspar ML, Marcos MA.
Crit Rev Immunol. 1998;18(6):485-501
1996
- Signal transduction: specificity of growth factors explained by parallel distributed processing.
Schamel WW, Dick TP.
Med Hypotheses. 1996 Sep;47(3):249-55. - The specificity of association of the IgD molecule with the accessory proteins BAP31/BAP29 lies in the IgD transmembrane sequence.
Adachi T, Schamel WW, Kim KM, Watanabe T, Becker B, Nielsen PJ, Reth M.
EMBO J. 1996 Apr 1;15(7):1534-41.
1995
- Molecular evolution of transfer RNA from two precursor hairpins: implications for the origin of protein synthesis.
Dick TP, Schamel WA.
J Mol Evol. 1995 Jul;41(1):1-9.
