Continuous microfluidic DNA extraction using phase-transfer magnetophoresis
11.10.2010
Karle M, Miwa J, Czilwik G, Auwärter V, Roth G, Zengerle R, von Stetten F.
Lab Chip 10(23):3284-90
We present the first continuous microfluidic platform for DNA extraction from cell lysate. Using magnetic beads, DNA is continuously transported across interfaces between co-flowing extraction reagents. The turnover time was 2 minutes with a sample flow rate of 0.75 µl/s and an eluate flow rate of 0.35 µl/s. DNA recovery yielded 147 % compared to off-chip batch-wise reference extractions. Possible applications are seen in biological safety and environmental monitoring or bioprocess control.
