Cooperation of Stop-Transfer and Conservative Sorting Mechanisms in Mitochondrial Protein Transport
17.06.2010
Bohnert M, Rehling P, Guiard B, Herrmann JM, Pfanner N, van der Laan M.
Curr Biol 20(13):1227-32
We dissected the membrane insertion of a multispanning ABC transporter. The N-terminal membrane domain was laterally released from the presequence translocase of the inner membrane
(TIM23 complex) by a stop-transfer mechanism, whereas the subsequent domain was imported via the matrix heatshock protein 70 (mtHsp70) motor and exported by the oxidase assembly (OXA) translocase. These observations lead to an unexpected solution to the controversial debate about mitochondrial preprotein sorting.
