Identification and characterization of NAGNAG alternative splicing in the moss Physcomitrella patens
28.04.2010
Sinha R, Zimmer AD, Bolte K, Lang D, Reski R, Platzer M, Rensing SA, Backofen R.
BMC Plant Biol 10:76
Alternative splicing (AS) involving tandem acceptors separated by three nucleotides (NAGNAG) is an evolutionarily widespread class of AS. We performed genome-wide identification of NAGNAG AS in the model plant Physcomitrella patens. Using evidence from Sanger and 454 sequencing we trained a classifier that was shown to predict 64% of splice sites correctly. Our results suggest that the mechanism behind NAGNAG AS in plants is similar to that in animals and is largely dependent on the splice site and its immediate neighbourhood.
