Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA)
12.01.2010
Lutz S, Weber P, Focke M, Faltin B, Hoffmann J, Müller C, Mark D, Roth G, Munday P, Armes N, Piepenburg O, Zengerle R, von Stetten F.
Lab Chip 10(7):887-93
We demonstrate a lab-on-a-foil system for fully automated analysis of DNA based on isothermal recombinase polymerase amplification. The system consists of a foil-based centrifugal microfluidic cartridge structured by blow-molding including prestored liquid and dry reagents, and a commercially available analyzer for incubation and real-time detection. The system is characterized by detection of the antibiotic resistance gene mecA. The limit of detection is < 10 copies within 15 minutes.
