Biogenesis of mitochondrial {beta}-barrel proteins: the POTRA domain is involved in precursor release from the SAM complex
16.06.2011
Stroud DA, Becker T, Qiu J, Stojanovski D, Pfannschmidt S, Wirth C, Hunte C, Guiard B, Meisinger C, Pfanner N, Wiedemann N.
Mol Biol Cell. 2011; 22(16):2823-33
We established a system to monitor the interaction of chemical amounts of ?-barrel precursor proteins with the SAM complex of wild-type and mutant yeast in isolated mitochondria.We report that the SAM complex lacking the POTRA domain of Sam50 efficiently binds ?-barrel precursors but is impaired in the release of the precursors. These results indicate that the POTRA domain of Sam50 is not essential for recognition of ?-barrel precursors but functions in a subsequent step to promote the release of precursor proteins from the SAM complex.
