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Exploring Cutting-Edge Biological Signalling Research in Freiburg

The Lord Mayor of Freiburg, Martin Horn, recently embarked on a visit to the renowned Signalhaus Freiburg, a symbol of research excellence housing the Clusters of Excellence BIOSS (Biological Signalling Studies) and CIBSS (Centre for Integrative Biological Signalling Studies). Accompanied by Ms....

2 new DFG research units at the University of Freiburg

The research units are aiming to reveal the potential of enzymatic catalysis and investigate the biological mechanisms that underlie bladder cancer.

First joint Open Day of CIBSS and BIOSS

In June, the two Freiburg signalling research centres CIBSS and BIOSS gave visitors insights into current research topics.

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BIOSS

ErbB2-associated changes in the lysosomal proteome

14.06.2011

Nylandsted J, Becker AC, Bunkenborg J, Andersen JS, Dengjel J, Jäättelä M.

Proteomics. 2011; 11(14):2830-8

Proteomics online article

The biochemical characterization of lysosomes has so far depended on purification methods based on either density gradient centrifugations or magnetic purification of iron-loaded organelles. Owing to dramatic changes in lysosomal density and stability associated with lysosomal diseases and cancer, these methods are not optimal for the comparison of normal and pathological lysosomes. Here, we introduce an efficient method for the purification of intact lysosomes by magnetic immunoprecipitation with antibodies against the vacuolar-type H1-ATPase.