DICER-LIKE3 activity in Physcomitrella patens DICER-LIKE4 mutants causes severe developmental dysfunction and sterility
17.04.2012
Arif MA, Fattash I, Ma Z, Cho SH, Beike AK, Reski R, Axtell MJ, Frank W.
Mol Plant. 2012;5(6):1281-94
Trans-acting small interfering RNAs (ta-siRNAs) are plant-specific siRNAs contributing to gene expression and signalling during development by direct sitespecific cleavage of target RNAs. The DICER-LIKE 4 protein of P. patens (PpDCL4)
is essential for the biogenesis of 21 nucleotide ta-siRNAs. In mutants (?PpDCL4), offsized ta-siRNAs accumulated as the result of PpDCL3 activity and severe abnormalities throughout the development occurred that were reversed in
?PpDCL3/?PpDCL4 double-mutants.
