A gene responsible for prolyl-hydroxylation of moss-produced recombinant human erythropoietin
22.10.2013
Parsons J, Altmann F, Graf M, Stadlmann J, Reski R, Decker EL.
Sci Rep. 2013;3:3019
Plant-based systems are gaining acceptance as production platforms for biopharmaceuticals. However, existing differences in the post-translational modifications between plants and humans may affect quality and safety of the products. Here we describe the identification of a plant gene responsible for non-human prolyl-hydroxylation of human erythropoietin (EPO) recombinantly produced in the moss bioreactor. Targeted ablation of this gene abolished the undesired modification of moss-produced EPO.
