Analysis of interactions between proteins and fatty acids or cholesterol using a fatty acid/cholesterol pull-down assay

31.01.2013

Beck-García E, Beck-García K, Schlosser A, Schamel WW.

Anal Biochem. 2013;436(2):75-7

Anal Biochem.         online article

Specific interactions between proteins and the polar head groups of membrane lipids have been described previously, but less is known about the proteins binding to lipid acyl chains. The method described in this BIOSS publication allows identification of such interactions by using a fatty acid or cholesterol pull?down assay combined with a subsequent Western blot analysis or mass spectrometry to identify transmembrane and cytosolic proteins that bound preferentially to short or long acyl chains or to cholesterol.