Green Targeting Predictor and Ambiguous Targeting Predictor 2: the pitfalls of plant protein targeting prediction and of transient protein expression in heterologous systems
05.08.2013
Fuss J, Liegmann O, Krause K, Rensing SA.
New Phytol. 2013 Dec;200(4):1022-33
The challenges of plant protein targeting prediction are the existence of dual subcellular targets and the bias of experimentally confirmed data towards few and mostly nonplant model species. We introduce two new targeting tools, GTP and ATP2, and conclude that targeting signals of divergent plant species exhibit differences, calling for custom in silico and in vivo approaches when aiming to unravel the actual distribution patterns of proteins within a plant cell.
