Leukocyte enrichment based on a modified pinched flow fractionation approach

08.10.2012

Cupelli C, Borchardt T, Steiner T, Paust N, Zengerle R, Santer M

Microfluid Nanofluid 2013;14(3-4):551-563

A simple and robust design for a passive hydrodynamic cell sorter based on pinched flow-field fractionation is presented and analyzed. Two principal layouts of the sorter are discussed and investigated experimentally as well as numerically based on the dissipative particle dynamics (DPD) method. Experimentally, design 1 approximately sorts 87 % of the erythrocytes to their designated outlet, while 100 % of the leukocytes branch correctly. This also holds for design 2 differing merely in the direction of the outlet for erythrocytes, but here only 69 % of the red blood cells are redirected to the designated outlet.

BIOSS

Leukocyte enrichment based on a modified pinched flow fractionation approach