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Exploring Cutting-Edge Biological Signalling Research in Freiburg

The Lord Mayor of Freiburg, Martin Horn, recently embarked on a visit to the renowned Signalhaus Freiburg, a symbol of research excellence housing the Clusters of Excellence BIOSS (Biological Signalling Studies) and CIBSS (Centre for Integrative Biological Signalling Studies). Accompanied by Ms....

2 new DFG research units at the University of Freiburg

The research units are aiming to reveal the potential of enzymatic catalysis and investigate the biological mechanisms that underlie bladder cancer.

First joint Open Day of CIBSS and BIOSS

In June, the two Freiburg signalling research centres CIBSS and BIOSS gave visitors insights into current research topics.

Events

BIOSS

Targeted purification of SnAvi-tagged proteins.

29.05.2014

Schäffer U, Baumeister R, Schulze E.

Methods Mol Biol. 2014;1177:163-74.

Methods Mol Biol online article

Tandem affinity purifi cation (TAP) is a powerful technique to identify protein complex members. The modular composition of TAP-tags allows two sequential protein enrichment steps and thereby drastically reduces the amount of contaminants. Here, we describe the application of the SnAvi-tag—a TAP-tag useful in different expression systems. Due to its modular composition, this tag is multifunctional and facilitates among others the in vivo visualization of tagged proteins and their cell type specific activation.