Chloroplast FBPase and SBPase are thioredoxin-linked enzymes with similar architecture but different evolutionary histories
Gütle DD, Roret T, Müller SJ, Couturier J, Lemaire SD, Hecker A, Dhalleine T, Buchanan BB, Reski R, Einsle O, Jacquot JP.
Proc Natl Acad Sci U S A. 2016;113(24):6779-84
Proc Natl Acad Sci U S A. online article
The two enzymes, fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase), function in the unique CO2 fixation pathway in chloroplasts. Especially the SBPase enzyme plays an important role as a bottleneck protein whose activity is directly linked to plant productivity. We were able to crystallize both phosphatases from Physcomitrella patens and performed an extensive biochemical, structural and phylogenetic comparison of the two enzymes.