Monochrome multiplexing in polymerase chain reaction by photobleaching of fluorogenic hydrolysis probes.
Schuler F, Trotter M, Zengerle R, von Stetten F.
Anal Chem. 2016;88(5):2590-5
16.02.2016
Schuler F, Trotter M, Zengerle R, von Stetten F.
Anal Chem. 2016;88(5):2590-5
Multiplexing in polymerase chain reaction (PCR) is a technique widely used to save cost and sample material and to increase sensitivity. One of the most common methods to detect the different amplification products is the use of fluorogenic probes. To circumvent restrictions to the amplifiable target length, the sequence, or the melting temperature, we suggest a novel approach that uses different fluorophores with the same emission maximum. Discrimination is achieved by their different fluorescence stability during photobleaching.
