Label-free isolation and deposition of single bacterial cells from heterogeneous samples for clonal culturing
Riba J, Gleichmann T, Zimmermann S, Zengerle R, Koltay P.
Sci Rep. 2016 Sep 6;6:32837.
The isolation and analysis of single prokaryotic cells down to 1 μm and less in size poses a special challenge and requires micro-engineered devices to handle volumes in the picoliter to nanoliter range. Here, an advanced Single-Cell Printer (SCP) was applied for automated and label-free isolation and deposition of bacterial cells encapsulated in 35 pl droplets by inkjet-like printing. individual bacterial cells from a heterogeneous sample of E. coli and E. faecalis were isolated for clonal culturing directly on agar plates in user-defined array geometry.
