The Physcomitrella patens gene atlas project: large-scale RNA-seq based expression data
Perroud PF, Haas FB, Hiss M, Ullrich KK, Alboresi A, Amirebrahimi M, Barry K, Bassi R, Bonhomme S, Chen H, Coates JC, Fujita T, Guyon-Debast A, Lang D, Lin J, Lipzen A, Nogué F, Oliver MJ, Ponce de León I, Quatrano RS, Rameau C, Reiss B, Reski R, Ricca M, Saidi Y, Sun N, Szövényi P, Sreedasyam A, Grimwood J, Stacey G, Schmutz J, Rensing SA.
In the framework of its Gene Atlas project, the Joint Genome Institute selected P. patens as a flagship genome, opening the way to generate an RNA-seq expression atlas of ca. 100 samples. Analysis of the three most distinct and abundant P. patens growth stages allowed us to define both general transcriptional patterns and stage-specific transcripts. As an example of variation of physico-chemical growth conditions, we detail here the impact of ammonium supplementation under standard growth conditions on the protonemal transcriptome.
