Host cell proteome of Physcomitrella patens harbors proteases and protease inhibitors under bioproduction conditions

J Proteome Res.             online article

Host cell proteins are inevitable contaminants of recombinant biopharmaceuticals that negatively affect purity and yield of the product. A proteomic and bioinformatic analysis of supernatants from moss bioreactors revealed a complex regulatory network of proteases and protease inhibitors involved in extracellular signalling. A knockout of the most abundant extracellular protease was performed in an aGAL-producing strain, the first moss-made pharmaceutical successful in clinical trials, resulted in a significant decrease of extracellular proteolytic activity.