Chloroplasts require glutathione reductase to balance reactive oxygen species and maintain efficient photosynthesis
Müller-Schüssele SJ, Wang R, Gütle DD, Romer J, Rodriguez-Franco M, Scholz M, Buchert F, Lüth VM, Kopriva S, Dörmann P, Schwarzländer M, Reski R, Hippler M, Meyer AJ.
Plant J. 2020; 103(3):1140-1154
Balancing reactive oxygen species is vital for plant signalling. We report a viable knock-out of organellar glutathione reductase in the moss Physcomitrella patens, linking plastid glutathione redox potential to photosynthetic performance. A redox sensitive roGFP was used to compare the redox potential in mutants and wildtype. This video shows the dynamic response of stromal glutathione redox potential to light, as measured via confocal live-imaging of the redox-sensor Grx1-roGFP2 targeted to the plastid stroma (green=reduced sensor, red=oxidised sensor).
