Modul „Signaling in Tumor Cells – Functional Proteomic Studies“

Wahlmodul

2. Semester M Sc Biologie, jedes Sommersemester

Modulverantwortlicher: Apl. Prof. Dr. Gerald Radziwill

Dozent/innen

• Friedel Drepper
• Silke Oeljeklaus
• Gerald Radziwill
• Bettina Warscheid

Lecture:

The lecture will provide a comprehensive overview of signalling pathways in health and disease and functional proteomics strategies combined with bioinformatics approaches

• Protein kinases and phosphatases in signalling networks
• Oncogenes and tumour suppressors
• Signaling in health and disease
• Protein kinases as targets in tumour therapy
• Advanced technologies to study posttranslational protein modifications
• Phosphoproteomics
• Quantitative proteomics (SILAC)
• High resolution mass spectrometry
• Bioinformatics tools

Practical Exercises:

Students will gain broad practical knowledge in cell culture technology and functional proteomics methods to analyse signalling mechanisms

• Handling and cultivation of mammalian cells
• Transfection of mammalian cells
• Fluorescence Microscopy
• Expression and analysis of protein kinases
• Inhibition of signaling pathways in breast cancer cells
• Purification and detection of phosphoproteins
• MS-based analysis of phosphoproteins
• Protein-protein interactions: affinity chromatography-MS
• Bioinformatics approaches & data analysis

Seminar:

Discussion of latest trends & technologies in signalling and functional proteomics

Lernziele:

To acquire comprehensive knowledge and practical experience in signal transduction and functional proteomic approaches